| 莱芜猪和杜洛克猪不同部位肌肉H-FABP基因差异表达 |
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| 引用本文： | 朱荣生,呼红梅,王怀中,张印.莱芜猪和杜洛克猪不同部位肌肉H-FABP基因差异表达[J].华北农学报,2009,24(Z2). |
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| 作者姓名： | 朱荣生 呼红梅 王怀中 张印 |
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| 作者单位： | 山东省农业科学院畜牧兽医研究所,山东,济南,250100;山东省畜禽疫病防治与繁育重点实验室,山东,济南,250100 |
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| 基金项目： | 山东省农业良种工程项目，山东省农业科学院青年基金 |
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| 摘 要： | 为研究不同品种猪不同部位肌肉H-FABP基因表达差异,探讨莱芜猪肌内脂肪沉积的分子机理,本试验选择体重100 kg莱芜猪10头、杜洛克5头,采用荧光定量RT-PCR法测定H-FABP基因在莱芜猪和杜洛克猪背最长肌和半膜肌中的表达量.品种间背最长肌和半膜肌H-FABP基因mRMA的表达差异虽未达到显著水平,但莱芜猪背最长肌和半膜肌H-FABP基因的表达水平均高于杜洛克猪.莱芜猪和杜洛克猪背最长肌与半膜肌间H-FABP基因表达量差异显著(P<0.05).莱芜猪和杜洛克猪H-FABP基因mRNA表达量与肌内脂肪含量显著相关.
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| 关 键 词： | 莱芜猪 荧光定量RT-PER H-FABP基因 肌肉 |
Differential Expression of H-FABP Gene of Differrent Part Muscles in Laiwu Pigs and Duroc |
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| ZHU Rong-sheng,HU Hong-mei,WANG Huai-zhong,ZHANG Yin.Differential Expression of H-FABP Gene of Differrent Part Muscles in Laiwu Pigs and Duroc[J].Acta Agriculturae Boreali-Sinica,2009,24(Z2). |
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| Authors: | ZHU Rong-sheng HU Hong-mei WANG Huai-zhong ZHANG Yin |
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| Abstract: | This experiment was conducted to study the differential expression of H-FABP gene in differential muscles between Laiwu pigs and Duroc, for making clear development of fat in Laiwu pigs. Ten Laiwu pigs and seven Duroc weighting approximately 100 kg were chosen for the experiment. Expression of H-FABP at mRNA in LM and semimembranous muscle of Laiwu pigs and Duroc were measured by real time fluorescence RT-PCR,IMF content were measured. The result indicated that : Compared with Duroc,H-FABP gene expression of longissimus muscle and semimembranous muscle in Laiwu pigs was appreciably increased(P>0.05). H-FABP gene expression of LM was markably lower than semimembranous muscle in Laiwu pigs and Duroc (P<0.05). Correlation between H-FABP gene expression of LM and IMF content in Laiwu pigs Duroc was prominent. |
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| Keywords: | Laiwu pigs Real-time fluorescence RT-PCR H-FABP gene expression Mucsle |
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