| 小檗碱和壳聚糖抗蔬菜病原真菌活性测定及复合膜制备 |
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| 引用本文： | 阎春琦,葛喜珍,田平芳.小檗碱和壳聚糖抗蔬菜病原真菌活性测定及复合膜制备[J].北方园艺,2009(8). |
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| 作者姓名： | 阎春琦 葛喜珍 田平芳 |
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| 作者单位： | 1. 北京化工大学,生命科学与技术学院,北京,100029
2. 北京联合大学,生物化学工程学院,北京,100023 |
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| 基金项目： | 北京市科协金桥工程资助项目 |
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| 摘 要： | 研究了小檗碱和壳聚糖对几种常见蔬菜病原真菌的抑制活性,以及以小檗碱和壳聚糖为主料制备复合膜的方法,并测定了该膜的药物释放效果。试验表明:低浓度(0.234 mg/mL)小檗碱即可抑制辣椒炭疽病菌(Vermicularia capsici)等5种蔬菜病原真菌的生长。20 mg/mL浓度壳聚糖对番茄灰霉病菌(Botrytis cinerea)的抑制率高达65%,而对其余4种果蔬病原真菌也有一定的抑制作用。为了集成这2种天然化合物的优点,制备了小檗碱-壳聚糖复合膜,该膜具有缓释功能,在模拟外部环境(磷酸缓冲液,pH 6.8)条件下,20 d小檗碱累计释放率接近25%,提示其在果蔬贮藏抗菌中的应用价值。
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| 关 键 词： | 小檗碱 壳聚糖 复合膜 蔬菜病原真菌 |
Fungistasis of Berberine and Chitosan and Preparation of Composite Membrane |
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| YAN Chun-qi,GE Xi-zhen,TIAN Ping-fang.Fungistasis of Berberine and Chitosan and Preparation of Composite Membrane[J].Northern Horticulture,2009(8). |
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| Authors: | YAN Chun-qi GE Xi-zhen TIAN Ping-fang |
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| Institution: | 1.College of Life Science and Technology;Beijing University of Chemical Technology;Beijing 100029;China;2.Bioengineering College of Beijing Union University;Beijing 100023;China |
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| Abstract: | The antifungal activities of berberine and chitosan were studied by growth rate method.It was found that berberine of low concentration(0.234 mg/mL) could inhibit the mycelial growth of vegetable pathogenic fungi,including Alternaria solani,Vermicularia capsici,Botrytis cinerea,Fusarium oxysporum f.sp.cucumerium,and Fusarium oxysporam f.sp.melonis.Another natural compound,chitosan also inhibited their mycelial growth.Chitosan showed approximately 65% inhibition rate against B.cinerea.To integrate the two na... |
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| Keywords: | Berberine Chitosan Composite membrane Vegetable pathogenic fungi |
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