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| 11个猪种SLC6A14基因3个SNPs的群体遗传变异研究 | |
| 引用本文： | 杨广礼,任军,张淑红,刘孟洲,张志燕,黄路生.11个猪种SLC6A14基因3个SNPs的群体遗传变异研究[J].畜牧兽医学报,2010,41(1). |
| 作者姓名： | 杨广礼 任军 张淑红 刘孟洲 张志燕 黄路生 |
| 作者单位： | 1. 商丘师范学院生命科学系,商丘,476000;甘肃农业大学动物科学技术学院,兰州,730070 2. 江西农业大学,江西省动物生物技术重点开放实验室,南昌,330045 3. 商丘师范学院生命科学系,商丘,476000 4. 甘肃农业大学动物科学技术学院,兰州,730070 |
| 基金项目： | 国家自然科学基金，教育部博士学科点专项基金 |
| 摘 要： | 本研究旨在了解猪种的遗传变异、种群间的亲缘关系和遗传分化。以白色杜洛克×二花脸资源家系F0代的17头二花脸母猪和2头白色杜洛克公猪的DNA池为模板,通过直接测序在猪SLC6A14基因内识别4个SNPs,以3个突变位点(g.7944AT、c.1438GA、g.21063GT)为基础,通过PCR-RFLP技术,对11个中外猪种进行多态性检测。结果表明:在SLC6A14g.7944AT和g.21063GT2个突变位点上,所有检测猪种都出现变异,其中槐猪、二花脸猪在g.7944AT位点上变异显著(0.01P0.05);而里岔黑猪、槐猪、玉山黑猪、合作藏猪、八眉猪在g.21063GT内变异极显著(P0.01),以GT基因型为主,且二花脸猪中出现TT基因型;在SLC6A14c.1438GA突变位点上,3个欧洲猪种(长白、大白、杜洛克)没有出现变异,均为GG纯合型,而其他猪种在此位点上均存在变异;再根据11个猪种3个位点的多态性信息计算Nei氏遗传距离,由UPGMA法构建聚类图,说明中国地方猪种与外引猪种存在明显的遗传分化;由此得出中国地方猪种比外引猪种有高的遗传多样性和变异,且存在明显的遗传分化,品种间多数猪种符合品种地域分布和品种特性。 |
| 关 键 词： | 猪 SLC6A14基因 遗传变异 |
Research on Population Genetic Variation of Three SNPs from SLC6A14 Gene in Eleven Pig Populations |
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| YANG Guang-li,REN Jun,ZHANG Shu-hong,LIU Meng-zhou,ZHANG Zhi-yan,HUANG Lu-sheng.Research on Population Genetic Variation of Three SNPs from SLC6A14 Gene in Eleven Pig Populations[J].Acta Veterinaria et Zootechnica Sinica,2010,41(1). | |
| Authors: | YANG Guang-li REN Jun ZHANG Shu-hong LIU Meng-zhou ZHANG Zhi-yan HUANG Lu-sheng |
| Institution: | YANG Guang-li1,3,REN Jun2,ZHANG Shu-hong1,LIU Meng-zhou3,ZHANG Zhi-yan2,HUANG Lu-sheng2(1.Department of Life Science,Shangqiu Normal University,Shangqiu 476000,China,2.Jiangxi Provincial Key Laboratory for Animal Biotechnology,Jiangxi AgriculturalUniversity,Nanchang 330045,3.College of Animal Science ,Technology,Gansu Agricultural University,Lanzhou 730070,China) |
| Abstract: | The aim of this research was to understand the population genetic variation and relation-ship, genetic differentiation between populations. In this study, 4 SNPs were identified in the porcine Solute Carrier Family 6 Member 14 (SLC6A14)by directly sequencing DNA pools from 19 founder animals(2 White Duroc boars and 17 Erhualian sows)of a White Duroc×Erhualian re-source population. The three mutation sites(c. 1438G>A, g. 7944A>T, g. 21063G>T)were chosen and used for detecting the polymorphism in 11 pig breeds by PCR-RFLP method. The re-sults showed that 11 pig breeds presented varation at the g. 7944A>T and g. 21063G>T sites in the SLC6A14. Moreover Huai and Erhualian pigs have dominant varation at g. 7944A>T site in the SLC6A14(0.01 |
| Keywords: | SNPs pig SLC6A 14 gene single nucleotide polymorphisms genetic variation |
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