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毛白杨种内过氧化物同工酶变异
引用本文:杨自湘,顾万春,李玲.毛白杨种内过氧化物同工酶变异[J].林业科学研究,1990,3(4):335-340.
作者姓名:杨自湘  顾万春  李玲
作者单位:中国林业科学研究院林业研究所 (杨自湘,顾万春),中国林业科学研究院林业研究所(李玲)
摘    要:用聚丙烯酰胺凝胶电泳分离了105份毛白杨的过氧化物同工酶,经三年重复,共获8种酶谱,表现了毛白杨种内遗传多型性。第一种酶谱是毛白杨种的主要酶谱型,占全部试材的85.7%,同属一种酶谱型的材料,其形态性别及生长习性都有明显差异,曾被定为新种、变种或变;不同酶谱材料的形态分歧应晚于酶分子水平的分歧。将8种酶谱的相似系数值进行聚类,第Ⅰ、Ⅱ、Ⅲ、Ⅳ类酶谱因发生较早可聚为一类,此类是毛白杨的主要类。第Ⅴ、Ⅵ、Ⅶ、Ⅷ种酶谱是近代毛白杨与派内几个种进行多元化、多地、多次杂交的新类型。

关 键 词:毛白杨  过氧化物同工酶  酶谱相似系数  种内遗传多型性  形态差异
收稿时间:1989/8/30 0:00:00

INTRASPECIFIC ISOPEROXIDASE VARIATION OF POPULUS TOMENTOSA CARR
Yang Zixiang,Gu Wanchun and Li Ling.INTRASPECIFIC ISOPEROXIDASE VARIATION OF POPULUS TOMENTOSA CARR[J].Forest Research,1990,3(4):335-340.
Authors:Yang Zixiang  Gu Wanchun and Li Ling
Institution:The Research Institute of Forestry CAF
Abstract:The results of studies on plant breeding and biosystematics showed that P. tomentosa originated in hybrid. Taxonomists have made a classification of various varieties of P. tomentosa but couldn't get unified opinion.The genetic variation of 105 clones collected from 15 regions of 6 provinces in main distribution area of P. tomentosa was analysed by means of polyarclamid gel electrophoresis. 8 types of isoperoxidase pattern were obtained from them, which showed genetic polymorphism within P. tomentosa.The clones, which showed the characteristics of type 1, occupied 85.7% of the total. Their distribution areas coincide with the distribution areas of P. tomentosa. The clones which belong to type 1, type 3, or type 4 ppossess different morphology, phenology, growing habits, sex and distribution. They were nominated as new varieties in some books. The differentiation of isoperoxidase is eailier than differentiation of morphology,so using these differences to supplement morphological taxonomy can make complicated things simpler.In order to further analyse the relationships among the 8 types of isoperoxidase patterns, we adopted their SCIP. When similarity distance (similarity coefficents + similarity distance=1) is 0.23, the varieties of type 1 to type 4 can be grouped into one. They should be the major type of P. tomentosa. The varieties of type 5 to type 8 have distant relationships with P. tomentosa and they possibly occurred in modern times. Because P. tomentosa is self originated in hybrid, its mordern population should be the hybrid population derived from the crossing of P. tomentosa with many other species of sect. Leuce repeatedly.
Keywords:Populus tomentosa Carr  isoperoxidase  similarity coefficients of isoperoxidase patterns
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