| 桑树叶片愈伤组织的诱导及不定芽分化影响因素的研究 |
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| 引用本文： | 王彦文,黄艳红,路国兵,王军妮,牟志美.桑树叶片愈伤组织的诱导及不定芽分化影响因素的研究[J].蚕业科学,2006,32(2):157-160. |
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| 作者姓名： | 王彦文 黄艳红 路国兵 王军妮 牟志美 |
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| 作者单位： | 四川农业大学动物科技学院,雅安,625014;山东农业大学林学院,泰安,271018;山东农业大学林学院,泰安,271018 |
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| 基金项目： | 山东省农业良种产业化工程项目 |
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| 摘 要： | 通过正交试验,探讨了植物生长调节剂噻二唑苯基脲(Th id iazuron,TDZ)、α-萘乙酸(NAA)、桑品种、叶龄、叶位等5种因素对桑树叶片愈伤组织诱导的影响。筛选出了桑树叶片愈伤组织诱导的最佳条件为:21 d苗龄的陕305组培苗叶片,培养基MS+1.0 mg/L TDZ+0.2 mg/L NAA。在此条件下,陕305愈伤组织诱导率可达93.9%。对得到的陕305叶片愈伤组织进行了不定芽分化诱导,结果表明,最佳分化培养基为MS+3.0 mg/L 6-BA+0.1 mg/L IAA+2%果糖,愈伤组织不定芽分化率高达100%。
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| 关 键 词： | 桑树 噻二唑苯基脲 愈伤组织 不定芽分化 |
| 文章编号： | 0257-4799(2006)02-0157-04 |
| 修稿时间： | 2006年3月20日 |
Studies on Factors Affecting Callus Induction and Adventitious Buds Differentiation From Leaf Culture in Mulberry |
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| WANG Yan-Wen,HUANG Yan-Hong,LU Guo-Bing,WANG Jun-Ni,MU Zhi-Mei.Studies on Factors Affecting Callus Induction and Adventitious Buds Differentiation From Leaf Culture in Mulberry[J].Acta Sericologica Sinica,2006,32(2):157-160. |
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| Authors: | WANG Yan-Wen HUANG Yan-Hong LU Guo-Bing WANG Jun-Ni MU Zhi-Mei |
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| Abstract: | Using orthogonal experiment design,the effect of Thidiazuron(TDZ) and α-Naphthalencacetic acid(NAA) content in the medium, effect of the order of leaf,the age of leaf and the varieties of mulberry on the leaf callus induction was investigated.Meanwhile,the effect of 6-benzylaminopurine(BA),indole-3-acetic acid(IAA) and sugar on callus differentiation was also studied.The results showed that the best condition for callus induction is: age of the mulberry cultured in vitro,21 d;medium,MS(Murashige & Skoog medium)+1.0 mg/L TDZ+0.2 mg/L NAA;and that the medium for adventitious bud differentiation of the callus is: MS+3.0 mg/L 6-BA+0.1 mg/L IAA,fructose,2%.Under these conditions,the leaf callus induction rate and adventitious bud differentiation rate for Shan 305 reached 93.9% and 100% respectively. |
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| Keywords: | Mulberry Thidiazuron Callus Adventitious bud differentiation |
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