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| 番鸭"新鸭疫"病原中呼肠孤病毒的初步确证 | |
| 引用本文： | 余旭平,何世成,朱军莉,金俊杰,郑新添,沈琴芳,张秀亮.番鸭"新鸭疫"病原中呼肠孤病毒的初步确证[J].畜牧兽医学报,2005,36(8):846-850. |
| 作者姓名： | 余旭平 何世成 朱军莉 金俊杰 郑新添 沈琴芳 张秀亮 |
| 作者单位： | 1. 浙江大学动物科学学院,杭州,310029 2. 浙江大学动物科学学院,杭州,310029;湖南省兽医总站,长沙,410007 3. 浙江工商大学食品、生物与环境工程学院,杭州310025 4. 温州市农科院动物研究所,温州325006 |
| 基金项目： | 浙江省科技计划重点项目（011102120）;宁波市科技计划项目（2002D40015） |
| 摘 要： | 1999年底以来,在浙江省的青壮年番鸭中新爆发了主要表现为食欲下降、咳嗽、流涕、喙和蹼发绀、拉稀、死亡率很高的烈性传染病,病理剖解主要表现为肝脏肿大、质脆,个别病例有不规则的坏死斑;脾肿大、有的病例坏死呈花斑;胰有多量、点状的坏死灶;肠道黏膜常有环状出血灶等。据当地百姓的俗称,笔者暂时把这种疾病定名为番鸭“新鸭疫”。 |
| 关 键 词： | 番鸭 “新鸭疫” 病原 呼肠孤病毒 S1基因 症状 病理变化 |
| 文章编号： | 0366-6964（2005）08-0846-05 |
| 收稿时间： | 2004-07-01 |
| 修稿时间： | 2004-07-01 |
Primary Evidences of a Reovirus as Pathogen of an Newly-outbreak Disease of Muscovy Duck in China |
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| YU Xu-ping,HE Shi-cheng,ZHU Jun-li,JIN Jun-jie,ZHENG Xin-tian,SHEN Qin-fang,ZHANG Xiu-liang.Primary Evidences of a Reovirus as Pathogen of an Newly-outbreak Disease of Muscovy Duck in China[J].Acta Veterinaria et Zootechnica Sinica,2005,36(8):846-850. | |
| Authors: | YU Xu-ping HE Shi-cheng ZHU Jun-li JIN Jun-jie ZHENG Xin-tian SHEN Qin-fang ZHANG Xiu-liang |
| Abstract: | A so called "new duck disease" with the character of necrosis of spleen and pancreas broke out in flocks of muscovy ducks at the end of 1999 in Zhejiang province. Viruses with different conformation and size, one of which was similar to reovirus were found under electronic microscope in allantoic fluid for virus isolation. Three pairs of primers were designed and synthesized based on the published S1 sequences of muscovy duck reovirus (mDRV). Specific RT-PCR products were obtained from both diseased tissues and allantoic fluid for virus isolation. Cloning and sequencing of the fragment and BLAST analysis result further verified the existence and participation of mDRV in the disease. A 1 260 bp S1 full-length sequence of the virus (ZJ99) was further amplified and sequenced. Phylogenic analysis result based on S1 sequences showed that the ZJ99 was in the same group with mDRVs of the French strain and the Fujian strain, while it had some distance to the group of the chicken reoviruses and was far more distant to that of mammalian. On the other hand, the nucleotide sequence of S1 of the ZJ99 strain had a homology of 98% with that of Fujian strain (300 bp partial sequence), while only had 90% homology to that of French strain. It was speculated that mDRVs isolated from different area of China, which had some differences with French strains, were the same strain but spreading in different area. |
| Keywords: | new duck disease muscovy duck reovirus S1 segment |
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