| 环介导等温扩增技术检测酸乳中蜡样芽孢杆菌 |
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| 引用本文： | 周巍,张薇,刘亮,刘东,王赞,秦丽,赵勇,张岩.环介导等温扩增技术检测酸乳中蜡样芽孢杆菌[J].乳业科学与技术,2013(5):29-31. |
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| 作者姓名： | 周巍 张薇 刘亮 刘东 王赞 秦丽 赵勇 张岩 |
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| 作者单位： | [1]河北省食品质量监督检验研究院河北石家庄050051 [2]河北省食品质量监督检验研究院,河北石家庄050051 |
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| 摘 要： | 目的：建立快速准确检测酸乳中蜡样芽孢杆菌的方法.方法：根据蜡样芽孢杆菌hblA基因设计特异性引物,通过环介导等温扩增技术（LAMP）直接检测酸乳中蜡样芽孢杆菌,扩增产物电泳检测.结果：LAMP法检测酸乳中蜡样芽孢杆菌的特异性强,方法检出限6.4CFU/mL,人工污染检出限21CFU/mL.结论：LAMP法用于检测酸乳中蜡样芽孢杆菌的灵敏度高、耗时短,为酸乳中蜡样芽孢杆菌的快速检测提供了新的方法.
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| 关 键 词： | 酸乳 环介导等温扩增 检测 蜡样芽孢杆菌 |
Detection of Bacillus cereus in Yogurt by Loop-Mediated Isothermal Amplification Assay |
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| ZHOU Wei＇,ZHANG Wei,LIU Liang,LIU Dong,WANG Zan,QIN Li,ZHAO Yong,ZHANG Yan.Detection of Bacillus cereus in Yogurt by Loop-Mediated Isothermal Amplification Assay[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2013(5):29-31. |
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| Authors: | ZHOU Wei＇ ZHANG Wei LIU Liang LIU Dong WANG Zan QIN Li ZHAO Yong ZHANG Yan |
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| Institution: | 1. Hebei Institute of Food Quality Supervision Inspection and Research, Shijiazhuang 050051, China; 2. College of Food Science and Technology, Agricultural University of Hebei, Baoding 071000, China) |
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| Abstract: | Objective： A loop-mediated isothermal amplification （LAMP） assay was designed for rapid and accurate detection of Bacillus cereus in yogurt. Methods： Two pairs of oligonucleotide primers exclusively amplifying the hblA gene of Bacillus cereus were designed and Bacillus cereus could be directly detected by LAMP in yogurt, and the amplification products were detected by electrophoresis. Results： Detection of Bacillus cereus in yogurt by the LAMP assay was highly specific, the sensitivity was 6.4 CFU/mL, and the detection limit was 21 CFU/mL. Conclusion： The LAMP was fast, sensitive and useful for the detection of Bacillus cereus in yogurt. |
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| Keywords: | yogurt： loop-mediated isothermal amplification （LAMP）： detection： Bacillus cereus |
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