| 苏云金芽孢杆菌对松材线虫的杀线活性 |
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| 引用本文： | 徐华潮,徐金华,张立钦,林海萍.苏云金芽孢杆菌对松材线虫的杀线活性[J].中国生物防治,2010(1):85-89. |
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| 作者姓名： | 徐华潮 徐金华 张立钦 林海萍 |
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| 作者单位： | 浙江林学院林业与生物技术学院,临安311300; |
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| 基金项目： | 国家948项目(2450000042) |
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| 摘 要： | 在室内条件下,测定了17株苏云金芽孢杆菌Bacillus thuringiensis伴孢晶体蛋白对松材线虫Bursaphelenchus xylophilus的杀线活性。通过初筛,发现菌株020、RBT-200701的伴孢晶体蛋白对松材线虫有明显毒杀作用。在测试的浓度范围内（菌株020为6.37-203.9μg/ml;RBT-200701为13.7-438.8μg/ml）,菌株对松材线虫的校正死亡率随其浓度增大而提高;随时间延长杀虫效果提高。020菌株晶体蛋白24h、48h时对松材线虫的LC50、LC95分别为183.20、408.20μg/ml和80.132、25.29μg/ml;RBT-200701菌株伴孢晶体蛋白24、48h时对松材线虫的LC50、LC95分别为368.00、868.00μg/ml和82.73、382.73μg/ml。SDS-PAGE分析发现这2个菌株晶体蛋白的蛋白图谱不同。
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| 关 键 词： | 苏云金芽孢杆菌 松材线虫 伴孢晶体蛋白 松材线虫病 |
Nematicidal Activity of Bacillus thuringiensis to Bursaphelenchus xylophilus |
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| XU Hua-chao
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XU Jin-hua
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ZHANG Li-qin
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LIN Hai-ping.Nematicidal Activity of Bacillus thuringiensis to Bursaphelenchus xylophilus[J].Chinese Journal of Biological Control,2010(1):85-89. |
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| Authors: | XU Hua-chao XU Jin-hua ZHANG Li-qin LIN Hai-ping |
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| Institution: | XU Hua-chao,XU Jin-hua,ZHANG Li-qin,LIN Hai-ping(School of Forestry , Bio-technology,Zhejiang Forestry College,Lin\'an 311300,China) |
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| Abstract: | The nematicidal activity of parasporal crystal proteins from 17 Bacillus thuringiensis strains against Bursaphelenchus xylophilus was tested.The results showed B.thuringiensis strains 020 and RBT-200701 were obvious virulent to B.xylophilus.The correction mortality of B.xylophilus was linearly related with the concentrations of each strain.The LC50 and LC95 of strain 020 at 24 h,48 h were 183.20,408.20 μg/ml and 80.13,225.29 μg/ml,respectively.LC50 and LC95 of strain RBT-200701 at 24 h,48 h were 368.00,868.00 μg/ml and 82.73,382.73 μg/ml,respectively.SDS-PAGE analysis demonstrated that strain 020 and strain RBT-200701 were different in protein profiles. |
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| Keywords: | Bacillus thuringiensis Bursaphelenchus xylophilus parasporal crystal protein pine wilt disease |
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