| 高效液相色谱法测定奶粉中磷脂酰丝氨酸含量 |
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| 引用本文： | 岳虹,赵贞,高敏,吕海燕,刘丽君,李翠枝.高效液相色谱法测定奶粉中磷脂酰丝氨酸含量[J].乳业科学与技术,2014(3):13-15. |
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| 作者姓名： | 岳虹 赵贞 高敏 吕海燕 刘丽君 李翠枝 |
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| 作者单位： | 内蒙古伊利实业集团股份有限公司,内蒙古呼和浩特010010 |
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| 基金项目： | “十二五”国家科技支撑计划项目（2012BAK17B00） |
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| 摘 要： | 建立了奶粉中磷脂酰丝氨酸的高效液相色谱-蒸发光散射检测的测定方法。采用氯仿甲醇混合溶剂做为提取剂,Diol色谱柱上分离,正己烷-异丙醇-乙酸-三乙胺和异丙醇-水-乙酸-三乙胺的二元系统进行梯度洗脱。每100 g奶粉中磷脂酰丝氨酸添加量为50~150 mg时,回收率大于85%。定量限30 mg/100 g。该方法具有结果准确,操作简便,重复性好等优点,可用于奶粉中磷酯酰丝氨酸的检测。
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| 关 键 词： | 高效液相色谱法 蒸发光散射检测器 磷脂酰丝氨酸 |
HPLC Determination of Phosphatidylserine in Milk Powder |
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| YUE Hong,ZHAO Zheng,GAO Min,LV Hai-yan,LIU Li-jun,LI Cui-zhi.HPLC Determination of Phosphatidylserine in Milk Powder[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2014(3):13-15. |
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| Authors: | YUE Hong ZHAO Zheng GAO Min LV Hai-yan LIU Li-jun LI Cui-zhi |
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| Institution: | (Inner Mongolia Yili Industrial Group Co.Ltd., Hohhot 010010, China) |
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| Abstract: | A method for the determination of phosphatidylserine in milk powder by High performance liquid chromatography with evaporative light scattering detection （HPLC-ELSD） was developed.Samples were extracted with a mixture of chloroform and methanol.Chromatographic separation was performed on a Diol column by gradient elution using a binary mobile phase system consisting of hexane-isopropanol-acetic acid triethylamine and isopropanol-water-acetic acid-triethylamine.The recoveries of phosphatidylserine in milk powder at spiked levels between 50 and 150 mg/100 g were greater than 85％,and the limit of quantification was 30 mg/100 g.The proposed method has the advantages of accuracy,simplicity and high repeatability,and is applicable for the analysis of phosphatidylserine in milk powder. |
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| Keywords: | HPLC ELSD phosphatidylserine |
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