| 高效液相色谱-串联质谱法快速测定乳与乳粉中20种同化激素 |
| |
| 引用本文： | 蔡玮红,何敏恒,李秀英,黄金凤,杜伟锋,李佩斯,冼燕萍,侯向昶,郭新东.高效液相色谱-串联质谱法快速测定乳与乳粉中20种同化激素[J].乳业科学与技术,2014,37(3):8-12. |
| |
| 作者姓名： | 蔡玮红 何敏恒 李秀英 黄金凤 杜伟锋 李佩斯 冼燕萍 侯向昶 郭新东 |
| |
| 作者单位： | 广州质量监督检测研究院国家加工食品质量监督检验中心(广州),广东广州,510110 |
| |
| 基金项目： | 广东省质量技术监督局科技项目（2013PS04） |
| |
| 摘 要： | 建立了液相色谱-电喷雾串联质谱同时测定乳与乳粉中11种甾类和9种β-受体激动剂类同化激素的快速确证方法.样品经体积分数0.1％甲酸乙腈溶液提取,正己烷脱脂,HLB固相萃取柱净化后,以0.1％甲酸甲醇-0.1％甲酸水为流动相梯度洗脱,通过C8色谱柱分离,电喷雾正离子多反应监测模式检测.在优化条件下,20种目标物在0.05～50 μg/L范围内线性良好,相关系数均大于0.996;方法定量限(RSN=10)在0.1～1.0 μg/kg之间;不同添加水平回收率为52.8％～97.4％,相对标准偏差(n=6)为2.8％～9.6％.多种市售纯牛奶和乳粉的测定结果表明,该方法操作简单、测定结果准确,可用于乳与乳粉中甾类和β-受体激动剂类激素多残留的快速测定.
|
| 关 键 词： | 液相色谱-电喷雾串联质谱 甾类 β-受体激动剂 乳 乳粉 |
Quick Determination of 20 Anabolic Hormones in Milk and Milk Powder by LC-MS-MS |
| |
| CAI Wei-hong,HE Min-heng,LI Xiu-ying,HUANG Jin-feng,DU Wei-feng,LI Pei-si,XIAN Yan-ping,HOU Xiang-chang,GUO Xin-dong.Quick Determination of 20 Anabolic Hormones in Milk and Milk Powder by LC-MS-MS[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2014,37(3):8-12. |
| |
| Authors: | CAI Wei-hong HE Min-heng LI Xiu-ying HUANG Jin-feng DU Wei-feng LI Pei-si XIAN Yan-ping HOU Xiang-chang GUO Xin-dong |
| |
| Institution: | (Guangzhou Quality Supervision and Testing Institute, National Centre for Quality Supervision and Testing of
Processed Food (Guangzhou), Guangzhou 510110, China) |
| |
| Abstract: | A quick confirmative method for the determination of residual amounts of 11 anabolic hormones and 9 agonists in milk and milk powder by liquid chromatography-tandem mass spectrometry （LC-MS-MS） was developed.Samples were extracted with acetonitrile containing 0.1％ formic acid by volume.After lipid removal by liquid-liquid extraction with n-hexane,the purification of the extract was carried out on an Oasis HLB cartridge by solid-phase extraction.The analytes were separated on a C8 column employing a mobile phase composed of a mixture of methanol with 0.1％ formic acid and 0.1％ formic for gradient elution.The tandem mass spectral acquisition was done in the positive electrospray ionization mode utilizing multiple reaction monitoring.External standard method was applied for quantization.The results indicated that the limits of quantification （LOQs,RSN =10） for 20 target compounds ranged from 0.1 to 1.0 μg/kg,and the linear range was 0.05-50 μg/L with correlation coefficients larger than 0.996.The average recoveries at different spiked concentration levels ranged from 52.8％ to 97.4％ with RSD of 2.8％-9.6％ （n =6）.The proposed method has been applied for the rapid determination of real samples of milk and milk powder with simple operation and accurate results. |
| |
| Keywords: | liquid chromatography-tandem mass spectrometry （LC-MS-MS） anabolic hormones β-agonists milk milk powder |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《乳业科学与技术》浏览原始摘要信息 |
| 点击此处可从《乳业科学与技术》下载免费的PDF全文 |
|
