| 根结线虫16D10基因与花生FAD2基因RNAi载体构建 |
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| 引用本文： | 于洪涛,唐月异,王秀贞,李贵杰,王传堂.根结线虫16D10基因与花生FAD2基因RNAi载体构建[J].花生学报,2011,40(1):1-5. |
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| 作者姓名： | 于洪涛 唐月异 王秀贞 李贵杰 王传堂 |
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| 作者单位： | 山东省花生研究所,山东青岛,266100 |
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| 基金项目： | 现代农业产业技术体系建设专项资金，青岛市科技发展计划 |
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| 摘 要： | 针对根结线虫16D10基因和花生△12脂肪酸脱氢酶(FAD2)基因的核酸序列设计了两条含有GUS内含子的RNA干扰结构序列.以pBS-T质粒为中间载体,将这段序列连接进植物转化载体pRI 101-AN中,获得针对两个基因的RNAi载体.经SalI和KpnI双酶切验证,目的序列已准确插入目标载体.此载体可用以培育抗根结线...
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| 关 键 词： | 根结线虫 花生 16D10基因 FAD2基因 RNA干扰 载体构建 |
Construction of RNAi Vector for Root-knot Nematode 16D10 Gene and Arachis hypogaea FAD2 Gene |
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| YU Hong-tao,TANG Yue-yi,WANG Xiu-zhen,LI Gui-jie,WANG Chuan-tang.Construction of RNAi Vector for Root-knot Nematode 16D10 Gene and Arachis hypogaea FAD2 Gene[J].Journal of Peanut Science,2011,40(1):1-5. |
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| Authors: | YU Hong-tao TANG Yue-yi WANG Xiu-zhen LI Gui-jie WANG Chuan-tang |
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| Institution: | (Shandong Peanut Research Insititute,Qingdao 266100,China) |
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| Abstract: | Two RNAi sequences with a GUS intron were designed and synthesized on the basis of nucleotide sequences of root-knot nematode（RKN） 16D10 gene and Arachis hypogaea Δ12 fatty acid desaturase（FAD2） gene.The RNAi sequences were ligated into pRI 101-AN plasmid,a plant transformation vector,via pBS-T vector.Target sequence was confirmed to be inserted into the vector correctly by double digestion with SalⅠand KpnⅠ.The RNAi constructs can be used to breed high oleate/RKN resistant peanut varieties. |
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| Keywords: | root-knot nematode Arachis hypogaea 16D10 FAD2 RNA interference vector construction |
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