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外源茉莉酸甲酯诱导小麦抗条锈病的研究
引用本文:蔚慧欣,高 利,刘太国,刘 博,康晓慧,陈万权.外源茉莉酸甲酯诱导小麦抗条锈病的研究[J].植物病理学报,2016,46(2):190-197.
作者姓名:蔚慧欣  高 利  刘太国  刘 博  康晓慧  陈万权
作者单位:西南科技大学生命科学与工程学院,绵阳 621010;
中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室,北京100193
基金项目:973计划(2013CB127701;2011CB100403);小麦产业技术体系(CARS-03);北京市科技新星计划(Z131105000413057)
摘    要:本文探讨了不同浓度茉莉酸甲酯(MeJA)对小麦抗病性的诱导作用,同时电镜观察了不同浓度MeJA对小麦条锈菌(Puccinia striiformis f.sp.tritici)与寄主细胞超微结构的影响。结果表明,2.00 mmol·L~(-1) MeJA对小麦条锈病的诱导抗性效果最好,可达到35.87%,而0.50 mmol·L~(-1)MeJA在小麦条锈菌夏孢子开始萌发4 h时呈显著抑制作用。经外源MeJA处理后,条锈菌的胞间菌丝、吸器母细胞和吸器的发育明显受到抑制,其中侵染位点的叶肉细胞呈抗性症状。因此,MeJA可使小麦幼苗产生诱导抗性从而提高其抗病性。

关 键 词:茉莉酸甲酯    小麦条锈病    诱导抗性    超微结构  

Preliminary study on resistance of wheat to stripe rust induced by methyl jasmonate
YU Hui-xin,GAO Li,LIU Tai-guo,LIU Bo,KANG Xiao-hui,CHEN Wan-quan.Preliminary study on resistance of wheat to stripe rust induced by methyl jasmonate[J].Acta Phytopathologica Sinica,2016,46(2):190-197.
Authors:YU Hui-xin  GAO Li  LIU Tai-guo  LIU Bo  KANG Xiao-hui  CHEN Wan-quan
Institution:College of Life science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China;
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Beijing 100193, China
Abstract:To understand the effects of different concentrations of methyl jasmonate (MeJA) on the resistance of wheat seeding against stripe rust caused by Puccinia striiformis f.sp. tritici (Pst), wheat seedling was inoculated with Pst after spraying with different concentrations of MeJA. The inducing effect was observed in the resistance to stripe rust pretreated with MeJA at the concentration of 2.00 mmol·L-1 with the effect reached to 35.87%, MeJA could inhibit the germination of urediniospore significantly at the concentration of 0.50 mmol·L-1 after 4 hours inoculation. Ultrastructure of intercellular hyphae, haustorium, and haustorial mother cells were significantly inhibited. A series of changes associated with resistance to infection were reported. Our results indicated that the resistance of wheat seedling against Pst was induced after MeJA treatment.
Keywords:methyl jasmonate  wheat stripe rust  induced resistance  ultrastructure  
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