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| 抗赤霉病普通小麦-大赖草易位系T5AS/5LrL的分子细胞遗传学鉴定 | |
| 引用本文： | 王林生,张雅莉.抗赤霉病普通小麦-大赖草易位系T5AS/5LrL的分子细胞遗传学鉴定[J].植物病理学报,2019,49(1):83-89. |
| 作者姓名： | 王林生 张雅莉 |
| 作者单位： | 河南科技大学农学院, 洛阳 471023; 洛阳市作物遗传改良与种质创新重点实验室,洛阳 471023 |
| 基金项目： | 国家自然基金项目(31501301);河南省科技攻关项目(182102110207);河南省教育厅自然科学研究项目(2011A180011) |
| 摘 要： | 大赖草作为小麦野生近缘植物,对赤霉病表现较好的抗性, 将其赤霉病抗性基因转入普通小麦, 对创新小麦赤霉病抗性种质有重要意义。本研究在获得抗赤霉病普通小麦-大赖草异附加系基础上, 采用1200R 60Co-γ射线处理小麦-大赖草二体附加系 DA5Lr花粉, 授予已去雄的普通小麦中国春,对其后代(M1)种子根尖细胞有丝分裂中期染色体进行GISH分析,获得了1株具有1条普通小麦-大赖草易位染色体的植株,让其自交,对自交后代中具有2条易位染色体植株的花粉母细胞减数分裂中期I进行观察,发现2条易位染色体形成了稳定的环状二价体,表明该植株为纯合体。利用顺序GISH-双色FISH分析,结合小麦D组专化探针Oligo-pAs1-2和B组专化探针Oligo-pSc119.2-2,进一步鉴定出该普通小麦-大赖草易位系为T5AS/5LrL,且筛选出了可追踪该易位系的3个EST-STS分子标记BE591127 、BQ168298和BE591737。赤霉病抗性鉴定结果表明,易位系T5AS/5LrL连续3年的病小穗率分别为7.69%、10.29%和8.66%,显著低于感病品种中国春和绵阳85-45。该易位系的育成为小麦赤霉病抗病性遗改良提供了新种质。 |
| 关 键 词： | 赤霉病 普通小麦-大赖草 易位系 病小穗率 |
| 收稿时间： | 2018-03-21 |
Molecular and cytogenetic identification of Triticum aestivum-Leymus racemosus translocation line T5AS/5LrL conferring resistance to Fusarium head blight |
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| WANG Lin-sheng,ZHANG Ya-li.Molecular and cytogenetic identification of Triticum aestivum-Leymus racemosus translocation line T5AS/5LrL conferring resistance to Fusarium head blight[J].Acta Phytopathologica Sinica,2019,49(1):83-89. | |
| Authors: | WANG Lin-sheng ZHANG Ya-li |
| Institution: | College of Agriculture, Henan University of Science and Technology, Luoyang 471023,Henan China; Luoyang Key Laboratory of Crop Genetic Improvement and Germplasm Innovation, Luoyang 471023, China |
| Abstract: | Leymus racemosus is a wild relative of wheat and has high resistance to wheat Fusarium head blight (FHB). The transfer of FHB resistance gene from L. racemosus to Triticum aestivum is of great significance for broadening the resistance of wheat germplasm. To obtain T. aestivum-L. racemosus translocation line with FHB resistance, the pollen of T. aestivum-L. racemosus disomic addition line DA5Lr was irradiated by 60Co-γ-rays 1200 R (100R·min-1) and then pollinated to emasculated T. aestivum cv. Chinese Spring. One plant with one T. aestivum-L. racemosus translocation chromosome was detected from the M1 plants by genomic in situ hybridization (GISH). This plant was self-pollinated, and the progenies with two translocation chromosomes were analyzed for chromosome pairing behavior at meiotic metaphase I of their pollen mother cells (PMCs). One ring bivalent was observed at meiotic metaphase I in one of the progenies, indicated that the plant was translocation homozygote. Using Oligo-pAs1-2 and Oligo-pSc119.2-2 as probe, the translocation line was further identified as T5AS/5LrL translocation line by C-banding and sequential GISH-FISH analysis. Three EST-STS markers, BE591127, BQ168298 and BE591737, were selected and could be used for tracing the translocation chromosome. Three years’ FHB resistance tests of T5AS/5LrL showed that the proportions of scabbed spikelets were 7.69%, 10.29% and 8.66%, respectively, which was obviously lower than that of susceptible varieties Chinese Spring and Mianyang 85-45 .The translocation line will provide a new source for FHB resistance in wheat breeding |
| Keywords: | Fusarium head blight Triticum aestivum-Leymus racemosus translocation line the proportion of scabbed spikelets |
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