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| 稻瘟菌群体中dsRNA的多样性及稻瘟菌菌株QSP5中病毒对寄主生物学性状影响的研究 | |
| 引用本文： | 唐利华,谢甲涛,程家森,付艳苹,张丽艳.稻瘟菌群体中dsRNA的多样性及稻瘟菌菌株QSP5中病毒对寄主生物学性状影响的研究[J].植物病理学报,2016,46(2):151-159. |
| 作者姓名： | 唐利华 谢甲涛 程家森 付艳苹 张丽艳 |
| 作者单位： | 华中农业大学湖北省作物病害监测和安全控制重点实验室,武汉 430070; 华中农业大学农业微生物学国家重点实验室,武汉 430070; 黑龙江省农业科学院中国科学院北方粳稻分子育种联合研究中心,哈尔滨 150086; 黑龙江省农业科学院博士后流动站,哈尔滨 150086 |
| 基金项目： | 黑龙江省科学基金面上项目(C2015032); 黑龙江省农业科学院杰出青年基金(2013) |
| 摘 要： | 真菌病毒在真菌中广泛存在,一些真菌病毒可以影响病原真菌营养生长、产孢和色素形成等性状。本文研究了真菌病毒在水稻稻瘟菌中的多样性以及其对寄主生物学性状的影响。采用单孢分离的方法从发病水稻叶片上分离获得了120个稻瘟菌菌株,其中约52%携带dsRNA片段。菌株QSP5被2个基因组为dsRNA的病毒即产黄青霉病毒1-C(Magnaporthe oryzae chrysovirus 1-C, MoCV1-C)和稻瘟菌病毒3(M. oryzae virus 3, MoV3)所侵染。对菌株QSP5进行单个的分生孢子纯化,获得了仅携带MoV3的菌株QSP5-9。菌株QSP5与QSP5-9的产孢量、菌丝形态以及菌落形态存在明显差异,经研究推测MoCV1-C与菌株QSP5 的产孢能力衰退、菌丝生长和产色素异常相关。同时对MoV3的稳定性进行了初步的研究。从稻瘟菌菌株QSP5获得了100个单孢分离物,通过对随机挑选的40个单孢分离物进行检测,结果表明这些分离物100%携带MoV3,只有部分单孢分离物携带MoCV1-C。另外,从稻瘟菌菌株QSP5-9获得了94个单孢分离物,对随机挑选的45个单孢分离物进行检测,结果表明这些分离物中均含有与菌株QSP5-9大小相同的dsRNA片段。根据以上结果推定MoV3在稻瘟菌菌株QSP5和QSP5-9及其无性后代中具有一定的稳定性。 |
| 关 键 词： | 稻瘟菌 真菌病毒 多样性 MoV3 MoCV1-C 稳定性 |
| 收稿时间： | 2015-05-03 |
Diversity of dsRNA mycoviruses in Magnaporthe oryzae and correlation relationship of the mycoviruses and biological characters of their host strain QSP5 |
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| TANG Li-hua,XIE Jia-tao,CHENG Jia-sen,FU Yan-ping,ZHANG Li-yan.Diversity of dsRNA mycoviruses in Magnaporthe oryzae and correlation relationship of the mycoviruses and biological characters of their host strain QSP5[J].Acta Phytopathologica Sinica,2016,46(2):151-159. | |
| Authors: | TANG Li-hua XIE Jia-tao CHENG Jia-sen FU Yan-ping ZHANG Li-yan |
| Institution: | Provincial Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China; Rice Molecular Breeding in Northern Chinese Academy of Sciences Joint Research Centre, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China; The Postdoctoral Workstation of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China |
| Abstract: | Mycoviruses are widespread in filamentous fungi and yeast; some of them are related to impaired growth, abnormal sporulation and pigmentation of their hosts. In this study, 120 M. oryzae isolates were obtained from rice blast lesions on leaves by using single-conidium isolation technique, and about 52% of these isolates contained dsRNA fragments. One of these strains QSP5 is infected by two mycoviruses, namely M. oryzae chrysovirus 1-C (MoCV1-C) and M. oryzae virus 3 (MoV3) according to their high similarity to M. oryzae chrysovirus 1-A (MoCV1-A) and M. oryzae virus 2 (MoV2). Besides, a single-conidium isolate QSP5-9, only infected by MoV3, was obtained from the conidia of strain QSP5. Comparative analysis of sporulation production, mycelial and colonial morphology between QSP5 and QSP5-9 revealed that MoCV1-C may be mainly rela-ted to abnormal sporulation, mycelial growth and pigmentation. Meanwhile, to test the stability of MoV3 in M. oryzae, 100 single-conidium isolates of M. oryzae strain QSP5 were obtained and MoV3 was detected in all the 40 randomly selected isolates, however, MoCV1-C only existed in part of these isolates. In addition, 94 single-conidium isolates of M. oryzae strain QSP5-9 were obtained and 45 randomly selected isolates were proved to carry the same dsRNA genome as strain QSP5-9. These results indicated that MoV3 is stable in M. oryzae. |
| Keywords: | Magnaporthe oryzae mycovirus diversity MoV3 MoCV1-C stability |
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