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| 基于全基因组序列的黄瓜绿斑驳花叶病毒重组和系统发育分析 | |
| 引用本文： | 许 帅,刘 群,耿 超,王 莹,田延平,贾 曦,李向东.基于全基因组序列的黄瓜绿斑驳花叶病毒重组和系统发育分析[J].植物病理学报,2016,46(3):320-327. |
| 作者姓名： | 许 帅 刘 群 耿 超 王 莹 田延平 贾 曦 李向东 |
| 作者单位： | 山东农业大学植物保护学院植物病理学系,泰安 271018; 山东省农科院,济南250000 |
| 基金项目： | 国家自然科学基金资助项目(31571984);教育部博士学科点基金资助项目(20123702110013) |
| 摘 要： | 通过RT-PCR扩增了黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)济南分离物(CGMMV-JN)的基因组片段。序列测定结果表明CGMMV-JN基因组全长6 424核苷酸(nt),5′-和3′-UTR分别为60和176 nt,含有4个ORF,分别编码129 kDa和186 kDa复制酶相关蛋白、29 kDa移动蛋白及17.4 kDa外壳蛋白。CGMMV-JN与另外29个CGMMV分离物全基因组核苷酸序列一致率为90.0%~99.7%。重组分析发现韩国KOM(AF417243)、以色列EC(KF155231)、印度(DQ767631)和我国河北的CHB(KJ658958)4个分离物在RdRp编码区存在重组。系统发育分析结果表明,这些分离物可分成3个组。选择压力分析结果表明cp基因处于正选择,其它基因处于负选择。本文研究的结果为黄瓜绿斑驳花叶病毒的监测及防控提供了理论依据。 |
| 关 键 词： | 黄瓜绿斑驳花叶病毒 重组 系统发育分析 全基因组序列 |
| 收稿时间： | 2015-04-22 |
Complete genomic sequences-based recombination and phylogenetic analysis of Cucumber green mottle mosaic virus |
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| XU Shuai,LIU Qun,GENG Chao,WANG Ying,TIAN Yan-ping,JIA Xi,LI Xiang-dong.Complete genomic sequences-based recombination and phylogenetic analysis of Cucumber green mottle mosaic virus[J].Acta Phytopathologica Sinica,2016,46(3):320-327. | |
| Authors: | XU Shuai LIU Qun GENG Chao WANG Ying TIAN Yan-ping JIA Xi LI Xiang-dong |
| Institution: | Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai′an 271018, China; Shandong Academy of Agricultural Sciences , Jinan 250000, China |
| Abstract: | The complete genome of a Cucumber green mottle mosaic virus isolate collected from watermelon in Ji’nan (CGMMV-JN) was amplified by RT-PCR. Sequencing analysis revealed that the full-length genome of CGMMV-JN is 6 424 nucleotides (nt) in length, of which the 5′-untranslated region (UTR) and 3′-UTR were 60 and 176 nt, respectively. The complete genome of CGMMV-JN contained four open reading frames (ORFs), which encode 129 kDa and 186 kDa RNA-dependent RNA polymerase (RdRp), 29 kDa movement protein, 17.4 kDa coat protein (CP), respectively. CGMMV-JN shared nucleotide identities of 90.0%-99.7% with other CGMMV isolates at the complete genome levels. Recombination analysis indicated that four isolates from Korea, Israel, India and China, respectively, presented recombination in ORF encoding RdRp. Phylogenetic tree analysis with complete genomic sequences demonstrated that the CGMMV isolates were clustered into three groups correlated with their geographical origin. Selection pressure analysis suggested that only CP ORF was under positive selection. Our study provided theoretical basis for the monitor and control of CGMMV. |
| Keywords: | Cucumber green mottle mosaic virus recombination phylogenetic analysis complete genomic sequence |
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