| 苹果茎痘病毒双重RT-PCR检测体系的建立及应用 |
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| 引用本文： | 胡国君,董雅凤,张尊平,范旭东,任芳,张梦妍.苹果茎痘病毒双重RT-PCR检测体系的建立及应用[J].植物病理学报,2019,49(3):428-432. |
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| 作者姓名： | 胡国君 董雅凤 张尊平 范旭东 任芳 张梦妍 |
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| 作者单位： | 中国农业科学院果树研究所, 国家落叶果树脱毒中心,兴城125100 |
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| 基金项目： | 中国农业科学院科技创新工程 |
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| 摘 要： | <正>病毒病是危害苹果(Malus domestica)的一类重要病害。已报道的苹果病毒种类很多,在我国发生普遍的主要有苹果褪绿叶斑病毒(Apple chlorotic leaf spot virus,ACLSV)、苹果茎沟病毒(Apple stem grooving virus,ASGV)、苹果茎痘病毒(Apple stem pitting virus,ASPV)、苹果花叶病毒(Apple mosaic virus,ApM V)以及苹果锈果类病毒(Apple scar skid viroid,ASSVd)~(1～3])。病毒或类病毒混合
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| 收稿时间： | 2018-07-11 |
Establishment and application of Apple stem pitting virus detection system using dual RT-PCR |
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| HU Guo-jun,DONG Ya-feng,ZHANG Zun-ping,FAN Xu-dong,REN Fang,ZHANG Meng-yan.Establishment and application of Apple stem pitting virus detection system using dual RT-PCR[J].Acta Phytopathologica Sinica,2019,49(3):428-432. |
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| Authors: | HU Guo-jun DONG Ya-feng ZHANG Zun-ping FAN Xu-dong REN Fang ZHANG Meng-yan |
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| Institution: | National Center for Eliminating Viruses from Deciduous Fruit Tree,Research Institute of Pomology,Chinese Academy of Agricultural Sciences,Xingcheng 125100,China |
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| Abstract: | Apple stem pitting virus (ASPV) has a worldwide distribution in pome fruit trees and leads to tremendous economic losses. A dual RT-PCR method was developed for ASPV detection from apple plants. The primer combination ASPV-F1/R1-F3R3 could simultaneously amplify two cDNA fragments covering two positions of viral genome, with the size 508 bp and 291 bp, respectively. The dual RT-PCR reaction was optimized at 60℃, using 0.6 uL ASPV-F1/R1 and 0.4 μL ASPV-F3R3 (10 mmol·L-1), and 2 μL cDNA. Moreover, the reaction system could be used for new leaves, phloem of shoot and in vitro apple plants and the amplification efficiencies of the tree materials were almost the same. The dual RT-PCR was simple, rapid and sensitive for ASPV detection. |
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| Keywords: | apple Apple stem pitting virus dual RT-PCR virus detection |
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