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| 番茄细菌性髓部坏死病病原的鉴定 | |
| 引用本文： | 佘小漫,汤亚飞,何自福,蓝国兵.番茄细菌性髓部坏死病病原的鉴定[J].植物病理学报,2017,47(2):180-186. |
| 作者姓名： | 佘小漫 汤亚飞 何自福 蓝国兵 |
| 作者单位： | 广东省农业科学院植物保护研究所,广州 510640; 广东省植物保护新技术重点实验室,广州 510640 |
| 基金项目： | 广东省科技计划项目(2014B070706017,2015A020209057);广东省农作物病虫害绿色防控技术研究开发中心建设项目 |
| 摘 要： | 2015年,在广东省广州市番茄种植区发生番茄髓部坏死病,从番茄病样中分离到一种细菌。随机选取5株细菌进行致病性测定,结果显示,这5株细菌均可侵染番茄植株产生典型的髓部坏死症状,与田间病株的症状相同。5株细菌的16S rDNA序列间同源性为99.9%~100%,且与菊苣假单胞菌(Pseudomonas cichori)MAFF211996、TIs01和IP1-05菌株的16S rDNA序列同源性最高,为99%。利用菊苣假单胞菌hrcR基因的特异引物Hrp1a/Hrp2a进行PCR,从这5株细菌的DNA中均能扩增出大小约897 bp的特异片段;这些片段序列间同源性为99%~99.9%,且与P.cichori 83-1菌株hrcR基因的序列同源性为99.9%。这些结果表明,引起广东番茄髓部坏死病的病原为菊苣假单胞菌(P.cichorii)。生理生化试验显示,该病原菌与文献报道的菊苣假单胞菌的生理生化特征均相吻合,进一步证实引起广东番茄髓部坏死病的病原为菊苣假单胞菌。 |
| 关 键 词： | 番茄髓部坏死病 菊苣假单胞菌 病原鉴定 16SrDNA 生理生化特征 |
Identification of the pathogen causing tomato bacterial pith necrosis in Guangdong |
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| SHE Xiao-man,TANG Ya-fei,HE Zi-fu,LAN Guo-bing.Identification of the pathogen causing tomato bacterial pith necrosis in Guangdong[J].Acta Phytopathologica Sinica,2017,47(2):180-186. | |
| Authors: | SHE Xiao-man TANG Ya-fei HE Zi-fu LAN Guo-bing |
| Institution: | Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China |
| Abstract: | In 2015, tomato pith necrosis disease was observed in tomato-growing area in Guangzhou of Guangdong province. Bacteria were consistently isolated from the diseased tomato samples. Pathogenicity test of 5 randomly selected bacterial isolates showed that the inoculated tomato plants exhibited the same symptoms as those observed in the field. 16S rDNA sequencing results indicated that the 5 isolates shared 99.9%～100% sequence identities with each other, and had a 99% sequence match to Pseudomonas cichorii strains MAFF 211996, TIs01 and IP1-05. Using the species-specific primer pair Hrp1a/Hrp2a of P. cichorii, an 897-bp hrcR gene-specific fragment could be amplified from the 5 isolates. Sequencing analysis suggested the amplified hrcR genes shared 99%～99.9% identities with each other, and had a 99.9% sequence identity with the hrcR of P. cichori 83-1. The physiological and biochemical tests further indicated these bacterial isolates were P. cichorii. All above results further support that tomato pith necrosis occured in Guangdong is caused by P. cichorii. |
| Keywords: | tomato pith necrosis Pseudomonas cichorii pathogen identification 16S rDNA physiological and biochemical characteristics |
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