| 番茄灰叶斑病原菌的鉴定及抗性种质资源的筛选 |
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| 引用本文： | 叶青静,阮美颖,王荣青,姚祝平,万红建,程远,李志邈,杨悦俭,周国治.番茄灰叶斑病原菌的鉴定及抗性种质资源的筛选[J].植物病理学报,2019,49(3):415-419. |
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| 作者姓名： | 叶青静 阮美颖 王荣青 姚祝平 万红建 程远 李志邈 杨悦俭 周国治 |
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| 作者单位： | 浙江省农科院蔬菜研究所,杭州 310021 |
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| 基金项目： | 浙江省重大科技专项(2016C02051);国家重点研发计划(2017YFD0101902);国家大宗蔬菜产业技术体系(CARS-23-G-44) |
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| 摘 要： | <正>番茄灰叶斑病是一种世界性病害,近年来,该病在北京、台湾、海南、山东和浙江等地均有发生,极大地限制了我国番茄生产的竞争力~(1])。该病害主要由Stemphylium spp.不同的4个种引起~(2])。本研究采用形态观察法、ITS和gpd序列分析法对从番茄病株分离得到的病原菌进行鉴定,并对番茄灰叶斑病抗性鉴定方法进行研究,以期为番茄灰叶斑病的抗病育种研究及其利用提供理论依据。
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| 收稿时间： | 2018-08-17 |
Identification of pathogen causing gray leaf spot on tomato and screening for resis-tant tomato germplasm |
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| YE Qing-jing,RUAN Mei-ying,WANG Rong-qing,YAO Zhu-ping,WAN Hong-jian,CHENG Yuan,LI Zhi-miao,YANG Yue-jian,ZHOU Guo-zhi.Identification of pathogen causing gray leaf spot on tomato and screening for resis-tant tomato germplasm[J].Acta Phytopathologica Sinica,2019,49(3):415-419. |
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| Authors: | YE Qing-jing RUAN Mei-ying WANG Rong-qing YAO Zhu-ping WAN Hong-jian CHENG Yuan LI Zhi-miao YANG Yue-jian ZHOU Guo-zhi |
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| Institution: | Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021,China |
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| Abstract: | Isolates of the gray leaf spot pathogen from diseased tomato plants were obtained by tissue-isolation method. Based on morphological characteristics, nucleotide sequences of ITS and gpd gene, the causal agents were identified as Stemphylium lycopersici. To screen available germplasm resources with gray leaf spot resistance, two inoculation methods, spray inoculation at the seedling stage and detached leaves identification method, were compared on two tomato materials 06-111A and T11-024A, and detached leaves identification method was selected to evaluate twenty-two tomato germplasm resources. The results indicated that eight germplasm were resistant, six were intermediate resistant and the others were susceptible. |
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| Keywords: | tomato gray leaf spot pathogen identification resistance screen |
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