| 西瓜嗜酸菌LuxR家族基因luxR_(4229)的功能研究 |
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| 引用本文： | 王铁霖,杨玉文,关 巍,赵廷昌.西瓜嗜酸菌LuxR家族基因luxR_(4229)的功能研究[J].植物病理学报,2016,46(2):198-206. |
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| 作者姓名： | 王铁霖 杨玉文 关 巍 赵廷昌 |
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| 作者单位： | 中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京 100193 |
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| 基金项目： | 公益性行业(农业)科研专项(201003066);国家西甜瓜产业技术体系(CARS-26);中国农业科学院创新工程 |
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| 摘 要： | 瓜类细菌性果斑病是葫芦科作物上的一种严重的世界性病害,其病原菌为西瓜嗜酸菌(Acidovorax citrulli)。本研究以野生型菌株Ac-5为研究对象,通过同源重组双交换的方法,构建了LuxR家族功能基因luxR_(4229)的全基因缺失突变株,并对突变株进行致病性、生物膜形成能力和运动能力等生物学特性测定。同时,qRT-PCR定量检测了鞭毛基因fliR和fliC以及III型分泌系统功能基因hrpE和hrcN的表达水平。结果显示:与野生型相比,突变株致病力明显降低,运动能力减弱,而生物膜形成能力增强。fliR、fliC、hrpE和hrcN基因在突变株中的表达量下调,luxR4229对fliR、fliC、hrpE和hrcN基因均为正调控。基因luxR_(4229)在对西瓜嗜酸菌致病力、运动能力及生物膜的形成具有重要调控作用。
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| 关 键 词： | 西瓜嗜酸菌 LuxR家族调控因子 luxR4229 |
Functional Analysis of luxR4229 , a LuxR family gene,in Acidovorax citrulli |
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| WANG Tie-lin,YANG Yu-wen,GUAN Wei,ZHAO Ting-chang.Functional Analysis of luxR4229 , a LuxR family gene,in Acidovorax citrulli[J].Acta Phytopathologica Sinica,2016,46(2):198-206. |
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| Authors: | WANG Tie-lin YANG Yu-wen GUAN Wei ZHAO Ting-chang |
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| Institution: | State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences, Beijing 100193,China |
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| Abstract: | Bacterial fruit blotch caused by Acidovorax citrulli is a world-wide disease that causes serious damages on the cucurbits plants. luxR4229 is a LuxR family member gene in A. citrulli Ac-5 isolated from watermelon. The luxR4229 deletion mutant was constructed by using two-step homologous recombination. The pathogenicity, biofilm formation and the swimming ability of the wild-type Ac-5 and its mutant strains were investigated. Meanwhile, the expression of the genes fliR and fliC encoding flagella proteins and the genes hrpE and hrcN involved in Type III secretion system were also determined by using qRT-PCR. The result showed that the mutant strain had lower pathogenicity, weaker swimming ability, but stronger ability of biofilm formation compared to its wild-type parent. The expressions of fliR, fliC, hrpE and hrcN genes were decreased in the mutant, indicating a positive regulation of luxR4229 to these genes. This study revealed that the luxR4229 gene plays an important regulatory role in the pathogenicity, swimming ability and biofilim formation of A. citrulli. |
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| Keywords: | Acidovorax citrulli LuxR-type regulator luxR4229 |
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