| 小麦幼苗内生菌多样性的宏基因组分析 |
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| 引用本文： | 赵芹,邓渊钰,李伟,孙海燕,夏云磊,陈怀谷.小麦幼苗内生菌多样性的宏基因组分析[J].植物病理学报,2017,47(3):313-324. |
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| 作者姓名： | 赵芹 邓渊钰 李伟 孙海燕 夏云磊 陈怀谷 |
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| 作者单位： | 江苏省农业科学院植物保护研究所,南京 210014 |
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| 基金项目： | 国家公益性行业(农业)项目(201503112) |
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| 摘 要： | 小麦是世界各地广泛种植的重要禾本科作物,在生长过程中与内生菌形成和谐共生的微生态体系,内生菌次生代谢产物具有抗病、防虫与促生等重要作用。宏基因组技术是研究环境微生物及内生菌多样性的重要手段。本研究首先富集纯化幼苗期小麦内生菌的宏基因组DNA,PCR扩增内生细菌的16S rDNA及内生真菌rDNA-ITS序列,构建了大肠杆菌克隆文库,选取阳性克隆测序。根据16S rDNA信息及系统进化树对内生细菌群初步分析,划分为9个分类操作单元(operational taxonomic unites,OTUs),主要包括γ-变形菌纲肠杆菌科(95.59%)、梭菌纲(1.47%)及不可培养细菌(2.94%),其中肠杆菌科菌群为优势菌。内生真菌rDNA-ITS克隆序列根据序列相似性和系统发育分析划归为13个OTUs,主要为不可培养真菌(37.50%)与子囊菌(62.50%),后者分属于半子囊菌纲(30.00%)、座囊菌纲(5.00%)与粪壳菌纲(27.50%)3个菌纲,其中不可培养真菌为优势菌。上述研究结果表明小麦幼苗内生菌遗传多样性分布较广,本研究为进一步挖掘利用小麦内生菌资源提供了基础信息。
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| 关 键 词： | 小麦幼苗 内生菌 宏基因组 多样性 系统发育分析 |
Analysis of endophytes diversity in wheat seedlings by metagenomic technology |
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| ZHAO Qin,DENG Yuan-yu,LI Wei,SUN Hai-yan,XIA Yun-lei,CHEN Huai-gu.Analysis of endophytes diversity in wheat seedlings by metagenomic technology[J].Acta Phytopathologica Sinica,2017,47(3):313-324. |
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| Authors: | ZHAO Qin DENG Yuan-yu LI Wei SUN Hai-yan XIA Yun-lei CHEN Huai-gu |
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| Institution: | Plant Protection Institute of Jiangsu Academy of Agricultural Science, Nanjing 210014, China |
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| Abstract: | Wheat is one of worldwidely important cereal crops, which lives together with its endophytes to form a balanced symbiotic and microecological system. The endophytes produced various secondary metabolites to protect host against fungi and pests and promote plant growth, etc. Metagenomic technology is an important method to investigate environmental microbes and endophytes. For investigating the diversity of endophytes in wheat seedlings, metagenome DNA of microbita from wheat seedlings were enriched and purified. The 16S rDNA of endophytic bacteria and rDNA-ITS of endophytic fungi were amplified by PCR, and then clonal libiraries were constructed and sequenced. According to 16S rDNA information and analysis of phylogenetic tree, endophytic bacteria were classified into 9 operational taxonomic units (OTUs) and mainly contained uncultured bacterium (2.94%), Enterobacteriaceae in Gammaproteobacteria (95.59%) and Clostridia (1.47%), of which Enterobacteriaceae bacteria were dominant. Based on sequence comparison and phylogenetic analysis of rDNA-ITS, clonal sequences of endophytic fungi were grouped into 13 OTUs, most of which were members of Ascomycota (62.50%)and uncultured fungi (37.50%), and the Ascomycota fungi were furthered identified as Saccharomycetes (30.00%), Dothideomycetes (5.00%) and Sordariomycetes (27.50%), thereinto uncultured fungi were dominant.This study showed a broad microbita spectrum of endophytic bacteria and fungi within wheat seedlings. This research provided basal information for mining and applying endophytes resources of wheat, and opened further insight into the great potential of wheat endophytics. |
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| Keywords: | wheat endophytes metagenome diversity phylogenetic analysis |
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