| 可可毛色二孢菌侵染后的烟草酵母双杂交cDNA文库构建 |
| |
| 引用本文： | 邢启凯,燕继晔,张 玮,刘 梅,富春元,李兴红.可可毛色二孢菌侵染后的烟草酵母双杂交cDNA文库构建[J].植物病理学报,2017,47(5):716-720. |
| |
| 作者姓名： | 邢启凯 燕继晔 张 玮 刘 梅 富春元 李兴红 |
| |
| 作者单位： | 北京市农林科学院植物保护环境保护研究所,北京100097 |
| |
| 基金项目： | 国家葡萄产业技术体系(CARS-30);北京市博士后工作经费资助项目(Project supported by Beijing Postdoctoral Research Foundation) |
| |
| 摘 要： | 正葡萄是世界上最重要的经济果树之一,据联合国粮食及农业组织统计,截止到2013年,中国葡萄栽培面积已达715.5万hm2,葡萄总产量超2 300万kg。长期以来,葡萄病害是影响葡萄产业健康发展的重要因素。近年来研究发现由葡萄座腔菌科真菌(Botryosphaeriaceae)引起的葡萄溃疡病(Botryosphaeria dieback)是葡萄上的主要枝干病
|
Construction of a yeast two-hybrid cDNA library from tobacco leaves infected by Lasiodiplodia theobromae |
| |
| XING Qi-kai,YAN Ji-ye,ZHANG Wei,LIU Mei,FU Chun-yuan,LI Xing-hong.Construction of a yeast two-hybrid cDNA library from tobacco leaves infected by Lasiodiplodia theobromae[J].Acta Phytopathologica Sinica,2017,47(5):716-720. |
| |
| Authors: | XING Qi-kai YAN Ji-ye ZHANG Wei LIU Mei FU Chun-yuan LI Xing-hong |
| |
| Institution: | Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China |
| |
| Abstract: | The occurrence of grapevine canker caused by pathogens of fungal family Botryosphaeriaceae is increasing in most grapevine growing areas worldwide. However, the mechanism of Botryosphaeriaceae-grapevine interaction is still in its infancy. In order to identify Botryosphaeriaceae resistance-related genes and targets of fungal effectors in grapevine, a yeast two-hybrid cDNA library of tobacco infected by Lasiodiplodia theobromae CSS-01s was constructed by SMART cDNA synthesis technology. The results showed that the titer of cDNA library was 1.2×108 CFU·mL-1. Results of PCR amplification of 24 clones randomly selected from the cDNA library revealed that the length of inserted cDNA fragments ranged from 350 bp to 2 000 bp, with a recombination rate of 100%. In addition, the average insert size of the cDNA library was 750 bp, indicating that this library is of high quality and could be applied for subsequent research. |
| |
| Keywords: | Botryosphaeriaceae Lasiodiplodia theobromae tobacco cDNA library yeast two-hybrid |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《植物病理学报》浏览原始摘要信息 |
| 点击此处可从《植物病理学报》下载免费的PDF全文 |
|
