| 利用RAPD研究松材线虫和拟松材线虫亲缘关系 |
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| 引用本文： | 汪来发,王扬,杨宝君,胡先奇,喻盛甫.利用RAPD研究松材线虫和拟松材线虫亲缘关系[J].植物病理学报,2001,31(3):225-229. |
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| 作者姓名： | 汪来发 王扬 杨宝君 胡先奇 喻盛甫 |
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| 作者单位： | 1 中国林业科学研究院, 北京 100091;2 云南农业大学, 昆明 650201 |
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| 基金项目： | 云南农业大学云南省植物病理学重点实验室开放基金资助项目 |
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| 摘 要： | 用RAPD技术对18个松材线虫株系、8个拟松材线虫株系基因组DNA多态性进行研究。在事先优化的条件下,用15个随机引物进行PCR扩增,一共扩增出165条谱带,多态性为91.5%。通过聚类分析,结果表明:松材线虫和拟松材线虫种间和种内都表现出很大的差异性,但种间差异大于种内差异,因而松材线虫和拟松材线虫应为2个独立的种。
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| 关 键 词： | 松材线虫 拟松材线虫 RAPD 亲缘关系 |
| 文章编号： | 0412-0914(2001)03-0225-05 |
| 修稿时间： | 1999年6月11日 |
STUDIES ON RELATIONSHIPS OF Bursaphelenchus xylophilus AND B. mucronatus BY RAPD |
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| WANG Lai fa ,WANG Yang ,YANG Bao jun ,HU Xian qi ,YU Sheng fu.STUDIES ON RELATIONSHIPS OF Bursaphelenchus xylophilus AND B. mucronatus BY RAPD[J].Acta Phytopathologica Sinica,2001,31(3):225-229. |
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| Authors: | WANG Lai fa WANG Yang YANG Bao jun HU Xian qi YU Sheng fu |
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| Institution: | 1 Chinese Academy of Forestry, Beijing 100091;2 Yunnan Agricultural University, Kunming 650201, China |
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| Abstract: | Random amplified polymorphic DNA (RAPD) technique was applied to assess DNA polymorphism among 18 strains of B. xylophilus and 8 strains of B .mucronatus. Amplifications with 15 primers under predetermined optimal reaction conditions gave 165 reproducible bands and the frequency of genetic diversity was 91.5%. By cluster analysis, the results showed that there was significant genetic diversity between B. xylophilus and B.mucronatus, which interspecific variations were more than intraspecific variations. Thus B. xylophilus and B.mucronatus are two distinct species. |
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| Keywords: | Bursaphelenchus xylophilus B mucronatus RAPD relationship |
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