| 海绵中氯丙酸脱氯微生物的分离及其初步研究 |
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| 引用本文： | 黄剑宇,信艳娟,张卫.海绵中氯丙酸脱氯微生物的分离及其初步研究[J].水产科学,2010,29(3). |
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| 作者姓名： | 黄剑宇 信艳娟 张卫 |
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| 作者单位： | 1. 中国科学院,大连化学物理研究所,海洋生物产品工程组,辽宁,大连,116023;中国科学院,研究生院,北京,100049
2. 中国科学院,大连化学物理研究所,海洋生物产品工程组,辽宁,大连,116023 |
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| 摘 要： | 以氯丙酸为单一碳源,通过富集培养从大连海域繁茂膜海绵中分离筛选得到139株氯丙酸降解菌。经过初步筛选得到35株降解能力较强的菌株。对这些菌株的耐盐试验表明,分离到的大部分菌株均具有较强的盐耐受能力,多数菌株为中度嗜盐菌。其中部分菌株可以在14.6%以上盐度培养基中生长,可能为极端嗜盐菌。多底物降解试验表明,大多数菌株均能降解多种底物,这些菌株可用于有机氯污染环境生物修复。同时也说明海绵对水体中有机氯污染物具有去除作用。
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| 关 键 词： | 氯丙酸降解 富集培养 中度嗜盐菌 海绵微生物 |
Isolation and Characterization of Haloacid Degrading Bacteria from Marine Sponge Hymeniacidon perleve |
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| HUANG Jian-yu,XIN Yan-juan,ZHANG Wei.Isolation and Characterization of Haloacid Degrading Bacteria from Marine Sponge Hymeniacidon perleve[J].Fisheries Science,2010,29(3). |
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| Authors: | HUANG Jian-yu XIN Yan-juan ZHANG Wei |
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| Institution: | 1.Marine Bioproducts Engineering Group;Dalian Institute of Chemical Physics;Chinese Academy of Sciences;Dalian 116023;China;2.Graduate School of the Chinese Academy of Sciences;Beijing 100049;China |
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| Abstract: | Microorganisms capable of producing 2-haloacid dehalogenase have been frequently isolated from the soil,but rarely from the sea.A total of 139 bacterial strains producing the 2-haloacid dehalogenase were enriched and isolated on agar with 2-chloropropionic acid as sole carbon source.Most of the strains showed high salinity tolerance,and were identified as moderately halophilic bacteria.Some of the strains showed the highest salinity tolerance up to 14.6% NaCl.Results of substrate specificity showed that mos... |
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| Keywords: | 2-chloropropionic acid degradation enrichment moderately halophilic bacterium sponge associated bacterium |
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