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鸡粪除氨菌株的分离、筛选与菌剂配制
引用本文:赵晓锋,于文清,田艳洪,刘文志,李鹏,隋文志.鸡粪除氨菌株的分离、筛选与菌剂配制[J].黑龙江农业科学,2012(6):85-87.
作者姓名:赵晓锋  于文清  田艳洪  刘文志  李鹏  隋文志
作者单位:黑龙江省农垦科学院农畜产品综合利用研究所,黑龙江佳木斯,154007
基金项目:黑龙江省科技厅科技攻关资助项目
摘    要:为减少鸡粪高温堆肥氨气的释放量,于2010~2011年,采用嗅味法进行初筛,采用硼酸吸收,稀硫酸滴定的方法进行复筛研究。结果表明:初筛得到具有除臭能力的菌株449株,并最终分离了细菌KB217、放线菌KA46和真菌KF21三个菌株,使鸡粪堆肥的氨气释放量分别降低了59.40%、55.05%和56.38%。初步鉴定细菌KB217为微球菌属,放线菌KA46为链霉菌属,真菌KF21为青霉属。拮抗试验证明3株菌之间无拮抗作用,菌株复合试验结果表明3株菌的混合制剂能使鸡粪堆肥的氨气释放量降低67.33%,并且缩短了氨气的释放时间。

关 键 词:微生物除臭  鸡粪  除氨气菌株  菌株复合  菌剂配制

Isolating and Screening of NH3 Removing Strains and Bacterial Agent Preparation of Chicken Manure
ZHAO Xiao-feng,YU Wen-qing,TIAN Yan-hong,LIU Wen-zhi,LI Peng,SUI Wen-zhi.Isolating and Screening of NH3 Removing Strains and Bacterial Agent Preparation of Chicken Manure[J].Heilongjiang Agricultural Science,2012(6):85-87.
Authors:ZHAO Xiao-feng  YU Wen-qing  TIAN Yan-hong  LIU Wen-zhi  LI Peng  SUI Wen-zhi
Institution:(Agricultural and Poultry Products Comprehensive Utilization Sciences Institute of Heilongjiang Academy of Land Reclamation Sciences,Jiamusi,Heilongjiang 154007)
Abstract:Aiming at reducing the release amount of NH3 of chicken manure during high temperature compost,primary screened by smell and secondary screening by the method of boric acid absorption,then titrated with dilute sulfuric acid were studied from 2010 to 2011.The result showed that primary screened 449 strains,and finally obtained three strains that could remove NH3,named KB217,KA46 and KF21,their removal rate were 59.40%、55.05% and 56.38%.Preliminary identified KB217 was micrococuss,KF21was penicillium,and KA46 was streptomyces.There was no antagonism between three strains,the result of strains complex experiment indicated that the agent mixed with three stains could decrease the release amount of NH3 by 67.33%,and shorten its release time.
Keywords:microbial deodorization  chicken manure  ammonia removing strains  strains complex  bacterial agent preparation
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