| 捻转血矛线虫Hc38基因部分功能域的原核表达与鉴定 |
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| 引用本文： | 陈南颖,何延华,王新华,薄新文,张兴亚.捻转血矛线虫Hc38基因部分功能域的原核表达与鉴定[J].中国预防兽医学报,2012,34(4):329-331. |
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| 作者姓名： | 陈南颖 何延华 王新华 薄新文 张兴亚 |
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| 作者单位： | 1. 石河子大学动物科技学院,新疆石河子832003;新疆兵团绵羊繁育生物技术重点实验室,新疆石河子832000
2. 新疆兵团绵羊繁育生物技术重点实验室,新疆石河子,832000 |
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| 基金项目： | 国家自然科学基金项目(30960281) |
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| 摘 要： | 为原核表达捻转血矛线虫Hc38基因的保守结构域,本研究参照GenBank中Hc38基因序列设计引物,扩增出Hc38基因的保守结构域序列,命名为HcP,将其克隆到表达载体pET32a中,并转化到大肠杆菌BL21中,用IPTG进行诱导表达.SDS-PAGE和western blot结果表明,HcP基因在大肠杆菌中高效表达,表达的重组蛋白分子量约为49.4 ku,并且表达产物能够与感染捻转血矛线虫的山羊血清产生特异的免疫印记条带,具有良好的反应原性.
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| 关 键 词： | 捻转血矛线虫 Hc38 原核表达 保守结构域 |
Expression and analysis of partial functional domains of Hc38 gene from Haemonchus contortus |
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| CHEN Nan-ying
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HE Yan-hua
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WANG Xin-hua
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BO Xin-wen
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ZHANG Xing-ya.Expression and analysis of partial functional domains of Hc38 gene from Haemonchus contortus[J].Chinese Journal of Preventive Veterinary Medicine,2012,34(4):329-331. |
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| Authors: | CHEN Nan-ying HE Yan-hua WANG Xin-hua BO Xin-wen ZHANG Xing-ya |
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| Institution: | 1.College of Animal Science and Technology,Shiheizi Uniersity,Shihezi 832003,China; 2.The Breed & Biotechnology Key Laboratory of Sheep in Reproduction & Construction Group of Xinjiang,Shihezi 832000,China) |
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| Abstract: | To express the conserved domain sequence of Haemonchus contortus Hc38 gene(HcP),the HcP sequence was amplified from H.contortus adult total RNA by RT-PCR with a pair of primers designed according to the cDNA available in GenBank.The HcP sequence was sub-cloned into pET32a and transformed into E.coli BL21 for expression in E.coli induced by IPTG.SDS-PAGE and western blot results showed molecular weight of the recombinant protein was about 50 ku,and recognized by positive sera against H.contortus,indicating that the recombinant protein possessed antigenic activity. |
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| Keywords: | Haemonchus contortus Hc38 expression conservative domain |
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