| 牙鲆家系4 个选育性状的测定与分析 |
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| 引用本文： | 王磊,胡灿灿,陈松林,田永胜,邓寒,李文龙,谢明树,牛余泽,庞仁谊,赵永伟.牙鲆家系4 个选育性状的测定与分析[J].中国水产科学,2015,22(4):620-629. |
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| 作者姓名： | 王磊 胡灿灿 陈松林 田永胜 邓寒 李文龙 谢明树 牛余泽 庞仁谊 赵永伟 |
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| 作者单位： | 1. 河南师范大学 水产学院, 河南 新乡 453007; 2. 中国水产科学研究院 黄海水产研究所, 农业部海洋渔业可持续发展重点实验室, 山东 青岛 266071 |
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| 基金项目： | 国家863 计划项目(2012AA10A408); 山东省自主创新专项(2013CXC80202); 山东省泰山学者工程专项; 2014 年度河南省高校科技创新团队支持计划(14IRTSTHN013). |
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| 摘 要： | 为了研究牙鲆(Paralichthys olivaceus)不同选育性状之间的相关性,2009年建立了38个牙鲆实验家系和1个养殖场对照组。选取其中33个家系和对照组进行鳗弧菌感染实验并对存活个体进行荧光标记后混合养殖在2个水泥池中,其中一个混养池在平均400日龄时暴发了淋巴囊肿病(Lymphocystis Disease,LD),LD发病率为62.4%,包括16个家系和对照组。从这些家系中筛选出4个发病率低于40.0%的抗病家系(F0939,F09104,F0915,F0908)。另外,在4~5月龄时,从每个家系中平均选取100尾鱼进行荧光标记后混合养殖,580日龄时测定这些家系的养殖存活率和体重,平均养殖存活率为32.0%,将7个养殖存活率高于55.0%的家系定义为高养殖存活率家系(F0908,F0975,F0990,F09125,F0927,F09119,F0905)。对牙鲆LD的抗病个体和易感个体的体重和全长进行卡方检验,发现抗病个体的体重和全长均显著高于易感个体(P0.01),同时分析牙鲆各个家系的LD发病率与发病时的体重和全长的相关性,结果显示牙鲆各个家系LD发病率与发病时体重和全长均呈强负相关(r–0.6)。对牙鲆家系鳗弧菌感染存活率、LD发病率、580日龄的养殖存活率和体重这4个性状两两之间进行相关性分析,发现LD发病率与580日龄的体重呈强负相关(r=–0.790);鳗弧菌感染存活率与养殖存活率之间为弱正相关(r=0.371);其他性状两两之间不存在显著相关性(–0.092≤r≤0.185)。本研究共筛选到了4个牙鲆LD抗病家系和7个高养殖存活率家系,并揭示了4个牙鲆选育性状之间的相关性,为培育速生多抗的牙鲆新品种提供了遗传材料和理论依据。
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| 关 键 词： | 牙鲆 家系 淋巴囊肿病 鳗弧菌 养殖存活率 体重 |
| 修稿时间： | 2015/7/30 0:00:00 |
Determination and analysis of 4 breeding traits in Japanese flounder(Paralichthys olivaceus) families |
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| WANG Lei,HU Cancan,CHEN Songlin,TIAN Yongsheng,DENG Han,LI Wenlong,XIE Mingshu,NIU Yuze,PANG Renyi,ZHAO Yongwei.Determination and analysis of 4 breeding traits in Japanese flounder(Paralichthys olivaceus) families[J].Journal of Fishery Sciences of China,2015,22(4):620-629. |
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| Authors: | WANG Lei HU Cancan CHEN Songlin TIAN Yongsheng DENG Han LI Wenlong XIE Mingshu NIU Yuze PANG Renyi ZHAO Yongwei |
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| Institution: | 1. College of Fisheries, Henan Normal University, Xinxiang 453007, China; 2. Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266 |
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| Abstract: | ) is an important commercially
cultured fish in China, Japan, andKorea. The rapid development of the Japanese
flounder aquaculture has led to problems with various diseases that havereduced
growth rates and caused huge economic loss. One way to address these problems
is to cultivate strains withcharacteristics such as high growth performance,
breeding survival rates, and disease resistance. For this purpose, 38families
and a control group were established in 2009. Four to five months after
hatching, 33 of the 38 families and thecontrol group were selected for exposure
to . The surviving individuals were marked with fluorescent-dye
and cultivated in two cement pools. Four hundred days after hatching,
lymphocystis disease (LD) broke out inone of the pools containing 16 families
and the control group. The average rate of incidence was 62.4%; those families(Families
8, 15, 39, and 104) with disease incidence below 40% were defined as
disease-resistant. At the same time, 100fish selected from each family were
marked with fluorescent-dye and cultivated in different cement pools. Roughly
580 dafter hatching, breed survival rate (580-BSR) and body weight (580-BW)
were determined. For the 38 families, the average580-BSR was 32.0%. There were
seven families (Families 5, 8, 27, 75, 90, 119, and 125) with a 580-BSR above55%,
and they were defined as high breed survival rate families. Notably, in 6 of
the 7 families, at least one parent wasderived from F0750 (Family 50
established in 2007), suggesting that individuals from F0750 may effectively
improve580-BSR. Chi-square tests indicated that body weight (400-BW) and total
length (400-TL) of the resistant group weresignificantly higher than those of
the susceptible group (
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| Keywords: | Paralichthys olivaceus family lymphocystis disease Vibrio anguillarum breeding survival rate body weight |
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