| 中国明对虾天门冬氨酸转氨酶基因的克隆及氨氮胁迫对其时空表达的影响 |
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| 引用本文： | 李少飞,何玉英,李吉涛,李健,刘萍,葛倩倩.中国明对虾天门冬氨酸转氨酶基因的克隆及氨氮胁迫对其时空表达的影响[J].中国水产科学,2014,21(6):1125-1133. |
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| 作者姓名： | 李少飞 何玉英 李吉涛 李健 刘萍 葛倩倩 |
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| 作者单位： | 1. 大连海洋大学 水产与生命学院, 辽宁 大连 116023; |
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| 基金项目： | 国家自然科学基金项目(31172401); 国家虾产业技术体系专项项目(CARS-47); 山东省自主创新专项项目(2013CX¬80202); 国家自然科学基金项目(31172401);公益性行业(农业)科研专项(201103034); 国家863计划项目 (2012AA10A409). |
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| 摘 要： | 利用RACE技术克隆获得中国明对虾(Fenneropenaeus chinensis)天门冬氨酸转氨酶GOT基因(FcGOT)。FcGOT 基因cDNA全长为1 910 bp, 其中, 开放阅读框1 284 bp, 编码427个氨基酸。同源性分析表明, 中国明对虾天门冬氨酸转氨酶GOT氨基酸序列与其他节肢动物高度保守, 与克氏原螯虾(Procambarus clarkii) 和桔粉蚧壳虫 (Planococcus citri) 的同源性分别为78%和73%。系统进化分析表明, FcGOT基因氨基酸序列与克氏原螯虾GOT聚为一支。组织表达分析发现FcGOT基因在肝胰腺、鳃、血细胞、肌肉、心脏、淋巴中均有表达, 其中肝胰腺中表达量最高。氨氮胁迫后, 荧光定量PCR分析结果表明, FcGOT基因在肝胰腺和鳃组织中的表达与对照组相比具有显著差异(P<0.05), 表明FcGOT基因在氨氮代谢方面具有重要的作用, 参与了中国明对虾机体的急性氨氮胁迫应答反应。
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| 关 键 词： | 国明对虾 天门冬氨酸转氨酶 基因克隆 基因表达 |
| 修稿时间： | 2015/8/4 0:00:00 |
Cloning and expression analysis of aspartate aminotransferase cDNA in Fenneropenaeus chinensis following ambient ammonia stresses |
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| LI Shaofei,HE Yuying,LI Jitao,LI Jian,LIU Ping,GE Qianqian.Cloning and expression analysis of aspartate aminotransferase cDNA in Fenneropenaeus chinensis following ambient ammonia stresses[J].Journal of Fishery Sciences of China,2014,21(6):1125-1133. |
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| Authors: | LI Shaofei HE Yuying LI Jitao LI Jian LIU Ping GE Qianqian |
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| Institution: | 1. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; |
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| Abstract: | is an important mariculture species in China. In aquaculture environments ammonia is a common toxic substance. higher frequencies of ammonia nitrogen toxicity in shrimps have been reported. Thereforeit is necessary to investigate ammonia metabolism by. As an important member of the AAT-like familythe enzyme aspartate aminotransferase (GOT) is involved in many aspects of ammonia metabolism including participating in inosine monophosphate transdeamination, detailed understanding of the regulation of GOT is of great significance. In this studywe successfully cloned the aspartate aminotransferase cDNA of -untranslated region(UTR) of 83 bp, protein exhibited typical AAT-like family features-phosphate binding sites suggesting that it belongs to the AAT-I superfamily. Homology analysis revealed that the amino acid sequence of was highly conserved with its homologs of other arthropods. The similarities of respectively. Phylogenetic analysis showed that . Tissue expression analysis showed that heart and lymph. The highest expression level of FcGOT in chinensis, 48 and 72 h after exposure to different concentrations of ammonia nitrogen. The expression of the gene was significantlydifferent compared with the control group (and the expression conditions differed between hepatopancreas and gill. In hepatopancreasps were exposed to 16and down-regulated after treatment with 4 mg/L ammonia-N for 48 h. In gill tissueambient ammonia stress and reached a peak at 12The reason for the discrepant expression between the two tissues may be that the gene expressions were affected by organizational distance. The results imply that metabolism and be involved in responses to acute ammonia stress. |
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| Keywords: | Fenneropenaeus chinensis aspartate aminotransferase gene cloning gene expression |
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