| 嗜水气单胞菌灭活疫苗免疫后大鲵外周血免疫指标的变化 |
| |
| 引用本文： | 杨星,刘文枝,肖汉兵,孟彦,高正勇,张辉,曾令兵.嗜水气单胞菌灭活疫苗免疫后大鲵外周血免疫指标的变化[J].中国水产科学,2014,21(3):621-628. |
| |
| 作者姓名： | 杨星 刘文枝 肖汉兵 孟彦 高正勇 张辉 曾令兵 |
| |
| 作者单位： | 1. 南京农业大学 无锡渔业学院, 江苏 无锡 214081; 2. 中国水产科学研究院 长江水产研究所, 湖北 武汉 430223; 3. 上海海洋大学, 上海 201306; 4. 浙江永强农业技术发展有限公司, 浙江 金华 321000 |
| |
| 基金项目： | 农业部公益性行业科研专项经费项目(201203086). |
| |
| 摘 要： | 以福尔马林灭活的嗜水气单胞菌(Aeromonas hydrophila)作为免疫原(F-AH), 通过腹腔注射免疫健康大鲵(Andrias davidianus), 分别于免疫后的第1、4、7、14、21、28天采集血液样品, 进行大鲵外周血液的血细胞计数与白细胞组成分析, 测定吞噬细胞的吞噬活性以及血清中和抗体效价, 于免疫28 d后进行攻毒感染试验。结果表明, 与对照组相比, 在免疫后第4、7、14和第21天, 免疫大鲵外周血中血细胞数量显著增加, 其中, 红细胞和白细胞数量均于第4天达峰值, 分别为7.83×107个/mL和6.74×106个/mL; 嗜中性粒细胞百分比也在第4天达峰值, 为28.60%, 单核细胞百分比在第7天达峰值, 为10.53%; 吞噬细胞活性显著提高, 且吞噬百分比和吞噬指数均在第4天达到最高值, 分别为34.09%和3.73。随后, 淋巴细胞百分比和中和抗体效价显著增加, 均在第21天达峰值, 分别为75.30%和1︰426.67。攻毒感染实验结果表明, 免疫组的相对免疫保护率为69.23%。由此可见, F-AH免疫原能够通过促进血细胞数量的增加、吞噬细胞吞噬活性增强以及特异性抗体的产生等方式提高大鲵的免疫保护力。
|
| 关 键 词： | 嗜水气单胞菌 灭活疫苗 大鲵 血细胞数量 吞噬活性 凝集抗体效价 相对免疫保护率 |
| 修稿时间： | 2015/7/16 0:00:00 |
Immune responses in the peripheral blood and protection of giantsalamander (Andrias davidianus) immunized with a killed Aeromonas hydrophila vaccine |
| |
| YANG Xing,LIU Wenzhi,XIAO Hanbing,MENG Yan,GAO Zhengyong,ZHANG Hui,ZENG Lingbing.Immune responses in the peripheral blood and protection of giantsalamander (Andrias davidianus) immunized with a killed Aeromonas hydrophila vaccine[J].Journal of Fishery Sciences of China,2014,21(3):621-628. |
| |
| Authors: | YANG Xing LIU Wenzhi XIAO Hanbing MENG Yan GAO Zhengyong ZHANG Hui ZENG Lingbing |
| |
| Institution: | 1. Wuxi Fisheries College, Nanjing Agricultural University, Jiangsu Wuxi 214081, China; 2.Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;3. College of Fisheries and Life Sciences, Shanghai Ocean Univer |
| |
| Abstract: | cells (F-AH) were used as an antigen to immunize healthy) via intraperitoneal injection. Peripheral blood samples were collected from immunized and control iant salamanders at 1, 4, 7, 21 and 28 d post-immunization immuneresponses by counting and classifying blood cells, determining the phagocytic percentage and index and serum agglutinating antibody titers. Compared to the control group, the number of erythrocytes and leucocytes in the peripheral blood of immunized animals increased significantly at 4, 7 d post-immunization, and reached a maximum value of 7.83×107/mL and 6.74×106/mL The percentage of neutrophils reached a maximum value of % at 4 d post-immunization and the number of monocytes reached a maximum value of % at 7 d post-immunization. The phagocytic percentage and phagocytic index increased significantly.at 4 d post-immunization, respectively. The number of lymphocyte and the serum agglutinating antibody titers increased significantly, and peaked at resulted in a relative percent survival in the immunized group of 69%. The results from this study demonst rated that F-AH enhanced the immunity of the iant salamander by increasing the number of erythrocytes and leucocytes in peripheral blood, improving the activity of phagocytes, the serum agglutinating antibodytiter and providing |
| |
| Keywords: | Aeromonas hydrophila inactivated vaccine Andrias davidianus haematocyte phagocytic activity agglutinating antibody titers relative percent survival |
|
| 点击此处可从《中国水产科学》浏览原始摘要信息 |
| 点击此处可从《中国水产科学》下载免费的PDF全文 |
|
