| 美洲鳗鲡致病性鲍曼不动杆菌的分离、鉴定及致病性分析 |
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| 引用本文： | 孙云,何明旺,张盼盼,李海平,曹贞洁,周永灿.美洲鳗鲡致病性鲍曼不动杆菌的分离、鉴定及致病性分析[J].水产学报,2020,44(9):1561-1571. |
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| 作者姓名： | 孙云 何明旺 张盼盼 李海平 曹贞洁 周永灿 |
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| 作者单位： | 海南大学,南海海洋资源利用国家重点实验室,海南 海口 570228;海南大学,海南省热带水生生物技术重点实验室,海南 海口 570228;海南大学,海南省热带水生生物技术重点实验室,海南 海口 570228 |
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| 基金项目： | 海南省自然科学基金(2019RC078)；海南省重大科技计划(ZDKJ2019011)；海南省南海名家青年项目 |
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| 摘 要： | 为确定海南某鳗鲡养殖场患病美洲鳗鲡大量死亡的原因,从患病美洲鳗鲡肝脏和脾脏中分离获得1株优势菌株AB01,回归感染证实该菌株为导致此次美洲鳗鲡患病的病原菌。经生理生化鉴定、16S rDNA基因序列比对及系统发育分析,判定菌株AB01为鲍曼不动杆菌。致病性分析结果显示,菌株AB01对美洲鳗鲡的半致死浓度为4.63×106CFU/mL。组织病理学观察显示,患病美洲鳗鲡的肝脏、脾脏均发生不同程度的病变,其中,肝脏中部分肝索排列紊乱,细胞肿胀,胞核溶解,胞质疏松,结构模糊,有大量大小不等的空泡;脾脏中胞质多处稀疏,伴有大面积损伤。药敏检测显示,鲍曼不动杆菌AB01对头孢噻吩、头孢唑啉、阿奇霉素、丁胺卡那霉素、利福平、依诺沙星、环丙沙星、氧氟沙星敏感,对氟苯尼考、氨苄西林等9种抗生素中度敏感,对氨曲南和四环素等20种抗生素有多重耐药性。
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| 关 键 词： | 美洲鳗鲡 鲍曼不动杆菌 分离鉴定 16SrDNA 药敏 |
| 收稿时间： | 2020/8/19 0:00:00 |
| 修稿时间： | 2020/9/10 0:00:00 |
Isolation, identification, and pathogenicity analysis of Acinetobacter baumannii in Anguilla rostrate |
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| SUN Yun,HE Mingwang,ZHANG Panpan,LI Haiping,CAO Zhenjie,ZHOU Yongcan.Isolation, identification, and pathogenicity analysis of Acinetobacter baumannii in Anguilla rostrate[J].Journal of Fisheries of China,2020,44(9):1561-1571. |
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| Authors: | SUN Yun HE Mingwang ZHANG Panpan LI Haiping CAO Zhenjie ZHOU Yongcan |
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| Institution: | State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China;Key Laboratory of Tropical Hydrobiology and Biotechnology, Hainan University, Haikou 570228, China |
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| Abstract: | In order to determine the cause of the large number of deaths of the sick American eel (Anguilla rostrate) in an aquaculture farm in Haikou (Hainan), one dominant strain (named AB01) was isolated from the liver and spleen of the diseased American eels. The dominant strain AB01 was identified as Acinetobacter baumannii based on the results from physiological and biochemical testing, sequence alignment of 16S rDNA gene and phylogenetic analysis. The challenge test in healthy American eels by artificial reentry indicated that A. baumannii was the pathogenic bacteria. Further analysis of pathogenicity showed that the semi-lethal concentration of A. baumannii (AB01) was 4.63×106 CFU/mL. Histopathological observation of liver from diseased Anguilla anguilla showed the arrangement of some hepatic cords was disordered, and the cells were swollen, together with dissolved nucleus, loose cytoplasm, and fuzzy structure. Moreover, a lot of vacuoles of different sizes were present in liver. In addition, spleen from diseased Anguilla rostrata presented sparse cytoplasm and extensive injury. The results of antimicrobial susceptibility test showed that A. baumannii (AB01) was resistant to many antibiotics, but was highly sensitive to cephalothin, cefazolin, azithromycin, amikacin, enoxacin, rifampicin, ciprofloxacin, ofloxacin, and medium sensitivity to nine antibiotics, such as florfenicol and so on. Moreover, A. baumannii (AB01) was resistant to twenty antibiotics, such as aztreonam and so on. Thus, this study provides scientific support for the prevention and control of the disease of the American eel. |
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| Keywords: | Anguilla rostrata Acinetobacter baumannii isolation and identification 16S rDNA antibiotic sensitivity test |
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