In this study, the 6R gene, which may be a functional gene involved in Andrias davidianus ranaviurs (ADRV) infection by bioinformatics analysis, was cloned and expressed. ADRV 6R about 711 bp was amplified by PCR, cloned into plasmid pMD18-T and the cloning plasmid containing ADRV 6R gene (pMD18-T-6R) was constructed. Then, the recombinant prokaryotic expression plasmid pET-32a-6R was constructed and sequenced. ADRV 6R gene was blasted with homologous sequences from other rana viruses of aquatic animals. The gene encodes a protein of 236aa with a predicted molecular mass of 27 kD. Amino acid sequence alignment of ADRV 6R showed a high homology with US22 family protein members in the viruses of aquaculture animals. The recombinant prokaryotic expression plasmid pET-32a-6R was transformed into DE3 and induced by IPTG. The expressed protein was purified with Ni-NTA His-Bind affinity chromatography, separated by different concentrations of imidazole eluent and analyzed by SDS-PAGE. A 45 kD fusion proteincontaining 27 kD interest protein and 18 kD his-tagged was obtained in accordance with the predicted molecular weight. These results provide useful experimental material for antibody preparation and study on ADRV-host interactions.