| 大黄鱼刺激隐核虫病组织中巨噬细胞移动抑制因子的检测分析 |
| |
| 引用本文： | 徐晓津,桑本红,覃映雪,邹文政,毛勇,邹峰,罗罡,苏永全.大黄鱼刺激隐核虫病组织中巨噬细胞移动抑制因子的检测分析[J].中国水产科学,2015,22(6):1227-1233. |
| |
| 作者姓名： | 徐晓津 桑本红 覃映雪 邹文政 毛勇 邹峰 罗罡 苏永全 |
| |
| 作者单位： | 1. 集美大学 水产学院, 福建省海洋渔业资源与生态环境重点实验室, 福建 厦门 361021;2. 厦门大学 海洋与地球学院, 福建 厦门 361005 |
| |
| 基金项目： | 福建省自然科学基金项目(2013J01140);2014年福建省海洋渔业资源与生态环境重点实验室科技项目(ZK2014004);2015年集美大学福建省大学生创新项目(201510390074);福建省研究生教育教学改革研究项目(闽教高[2015]11号,集大研[2015]19号);厦门南方海洋研究中心科技项目,海洋经济发展区域示范项目(14PYY050SF03;14PYY050SF03). |
| |
| 摘 要： | 由刺激隐核虫(Cryptocaryon irritans)感染引发的"白点病"为大黄鱼(Larimichthys crocea)养殖业带来重大损失,但仍未找到安全、有效的治疗方法。本研究应用免疫组织化学方法,对健康大黄鱼和患刺激隐核虫病大黄鱼肠、脾、头肾与肝组织中巨噬细胞移动抑制因子(macrophage migration inhibitory factor,MIF)蛋白进行检测,研究刺激隐核虫病大黄鱼组织中MIF的表达情况及其对预后的影响。结果表明,MIF在健康大黄鱼肠、脾、头肾、肝各组织中均无表达;MIF在刺激隐核虫病大黄鱼各组织中高表达,表达强度从弱到强依次是肠、肝、脾、头肾,MIF表达阳性率分别为54%、80%、86%、90%。推测MIF在大黄鱼刺激隐核虫病发病过程中发挥作用。
|
| 关 键 词： | 大黄鱼 巨噬细胞移动抑制因子 刺激隐核虫 免疫组织化学 |
| 修稿时间： | 2015/11/20 0:00:00 |
Detecting macrophage migration inhibitory factor in tissues of Larimichthys crocea with Cryptocaryon irritans disease |
| |
| XU Xiaojin,SANG Benhong,QIN Yingxue,ZOU Wenzheng,MAO Yong,ZOU Feng,LUO Gang,SU Yongquan.Detecting macrophage migration inhibitory factor in tissues of Larimichthys crocea with Cryptocaryon irritans disease[J].Journal of Fishery Sciences of China,2015,22(6):1227-1233. |
| |
| Authors: | XU Xiaojin SANG Benhong QIN Yingxue ZOU Wenzheng MAO Yong ZOU Feng LUO Gang SU Yongquan |
| |
| Institution: | 1. Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Fisheries College, Jimei University, Xiamen 361021, China;2. College of Ocean & Earth Sciences, Xiamen University, Xiamen 361005, China |
| |
| Abstract: | <i>Cryptocaryon irritans</i> is a holotrichous marine ciliate protozoan that causes "white spot disease", which is one of the most devastating parasites of <i>Larimichthys crocea</i> in Fujian Province. No safe drug strategy has been identified to prevent and cure "white spot disease". To investigate the expression and prognostic value of macrophage migration inhibitory factor (MIF) in <i>C. irritans</i> diseased <i>L. crocea</i> tissues, MIF expression was detected by immunohistochemistry of intestine, spleen, head kidney, and liver specimens of pathologically identified and healthy <i>L. crocea</i> tissues. None of the negative controls reacted, but MIF staining was intense in all <i>L. crocea</i> tissues infected with<i> C. irritans</i> disease. The MIF staining rates for the intestines, liver, spleen, and head kidney were 54%, 80%, 86% and 90%, respectively. The high MIF expression levels in diseased tissues indicate that MIF plays an important role in homeostasis of <i>C. irritans</i> infected with <i>L. crocea</i>. |
| |
| Keywords: | Larimichthys crocea macrophage migration inhibitory factor Cryptocaryon irritans immunohistohemistry |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国水产科学》浏览原始摘要信息 |
| 点击此处可从《中国水产科学》下载免费的PDF全文 |
|
