| 芹菜生长及黄酮类物质积累对PEG6000胁迫的响应 |
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| 引用本文： | 阎 君,于力,王 颖,王 虹,朱为民,朱龙英.芹菜生长及黄酮类物质积累对PEG6000胁迫的响应[J].中国农学通报,2018,34(25):45-51. |
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| 作者姓名： | 阎 君 于力 王 颖 王 虹 朱为民 朱龙英 |
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| 作者单位： | 上海市农业科学院设施园艺研究所/上海市设施园艺技术重点实验室,上海市农业科学院,上海市农业科学院设施园艺研究所/上海市设施园艺技术重点实验室,上海市农业科学院设施园艺研究所/上海市设施园艺技术重点实验室,上海市农业科学院设施园艺研究所/上海市设施园艺技术重点实验室,上海市农业科学院设施园艺研究所/上海市设施园艺技术重点实验室 |
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| 基金项目： | 国家自然科学青年基金“芹菜中芹菜素积累关键酶基因分析及分子标记开发与验证”(31601752)；上海市科技兴农项目“上海市绿叶蔬菜 产业技术体系”（沪农科产字2 号）；青海省重点研发与成果转化(2016-HZ-820)；上海市农业科学院攀高计划(PG141)；上海市农业科学院卓越团队 (ZY1601)。 |
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| 摘 要： | 目的]为了分析干旱处理对芹菜生长和黄酮类物质积累的影响。方法]本研究以芹菜品种‘申芹1号’为试验材料,采用聚乙二醇(PEG6000)室内水培法模拟干旱胁迫,研究轻度干旱(10%PEG 6000处理)、重度干旱(30%PEG 6000处理)胁迫下芹菜生长指标、渗透调节物质、抗氧化酶系统及黄酮类物质和相关代谢酶活性的变化。结果]轻度干旱处理下,芹菜生物量与对照无显著差异,脯氨酸和可溶性糖含量以及抗氧化酶活性显著升高,H2O2和O2ˉ.含量与对照无显著差异；苯丙氨酸解氨酶(PAL)、查尔酮合成酶(CHS)和查尔酮异构酶(CHI)活性显著升高,黄酮类物质芹菜素和毛地黄黄酮含量相比于对照显著上升,其叶片和叶柄中芹菜素含量分别比对照高出了36.05%和 40.35%,毛地黄黄酮含量分别比对照高出了34.39%和43.09%。重度干旱处理下,芹菜生物量显著低于对照,株高、地上部干重和地下部干重分别比对照降低52.60%、56.31%和144.44%,脯氨酸和可溶性糖含量与对照比差异不显著,抗氧化酶活性显著高于对照,其中CAT活性显著低于轻度干旱处理,H2O2和O2ˉ.含量显著升高；芹菜中黄酮类物质含量高于对照但不存在显著差异,CHS和CHI的酶活性显著低于轻度干旱胁迫。上述指标在叶片与叶柄的变化趋势基本一致。结论]由此可以得出,轻度干旱(10%PEG6000处理)在保持芹菜正常生长的前提下,能够促进芹菜黄酮类化合物的积累。
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| 关 键 词： | 谷子 谷子 硒 叶绿素荧光参数 SPAD |
| 收稿时间： | 2018/4/4 0:00:00 |
| 修稿时间： | 2018/5/16 0:00:00 |
Response of Growth and Flavonoids Accumulation in Celery Leaves to PEG6000 Stress |
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| Abstract: | The study aims to analyze the effect of drought treatment on the growth and accumulation of flavonoids in celery. The change of biomass, osmolyte, antioxidant enzyme system, key enzyme in flavonoids metabolize and flavonoid accumulation in celery variety‘Shenqin 1’are studied by solution culture under mild drought (10% PEG6000 treatment) and severe drought (30% PEG6000 treatment). In mild drought condition, compared with control, no significant difference in biomass and H2O2, O2ˉ·content was observed; proline and soluble sugar content, antioxidant enzyme activity, phenylalanine ammonia lyase (PAL), chalcone synthase (CHS) and chalcone isomerase (CHI) activity and apigenin and luteolin content increased significantly. Compared with those of control, the apigenin content in leaf blade and leaf stalk was increased by
36.05% and 40.35%, respectively, and luteolin content was increased by 34.39% and 43.09%, respectively. Under severe drought condition, compared with control, the biomass of celery was significantly decreased, the plant height, shoot dry weight and root dry weight reduced by 34.47% , 36.02% , 59.09% , respectively. Compared with control, no significant difference in proline and soluble sugar content was observed, H2O2, O2ˉ·content and antioxidant enzyme activity increased remarkably (CAT activity was significantly lower than mild drought treatment); there was no significant increase in apigenin and luteolin content, and CHS and CHI activity were significantly reduced compared with that under mild drought stress. The change of the indexes mentioned above was almost the same in leaf blade and leaf stalk. It is concluded that mild drought (10% PEG6000 treatment) could keep a normal growth and meanwhile promote the accumulation of flavonoids in celery. |
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| Keywords: | celery PEG6000 stress biomass flavonoids |
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