| 烟蚜传播黄瓜绿斑驳花叶病毒的可能性及方式 |
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| 引用本文： | 刘大成,洪璠璠,柳傲,杨中侠.烟蚜传播黄瓜绿斑驳花叶病毒的可能性及方式[J].中国农学通报,2018,34(26):134-139. |
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| 作者姓名： | 刘大成 洪璠璠 柳傲 杨中侠 |
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| 作者单位： | 湖南农业大学植物保护学院 长沙,湖南农业大学植物保护学院 长沙,湖南农业大学植物保护学院 长沙,湖南农业大学植物保护学院 长沙 |
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| 基金项目： | 湖南省长沙市烟草站项目“烟蚜斜纹夜蛾抗药性监测及”(201301)。 |
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| 摘 要： | 为确定烟蚜体内携带的是否为黄瓜绿斑驳花叶病毒（CGMMV），采用RT-PCR和序列测定方法对其进行鉴定。本研究通过采集湖南宁乡、郴州、浏阳三个地区的田间烟蚜，提取RNA后反转录成cDNA，并以cDNA作为模板，通过设计引物进行PCR扩增，摇菌后测序。测序结果显示，在烟蚜体内扩增出与黄瓜绿斑驳花叶病毒相似度为99%的RNA病毒。对包括该分离物在内的22个基因核苷酸序列进行同源性比较和系统进化树构建，并结合地域来源进行分析，结果表明烟蚜体内的黄瓜绿斑驳花叶病毒(CGMMV)来源于其携带的CGMMV。通过分析推测，烟蚜可传播黄瓜绿斑驳花叶病毒，且传毒方式为持久性。
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| 关 键 词： | 微生物学 微生物学 铀及伴生重金属 超富集植物 纤维素降解菌 |
| 收稿时间： | 2017/9/7 0:00:00 |
| 修稿时间： | 2018/1/14 0:00:00 |
The Possibility and Way of Myzus Persicae Spreading Cucumber Green Mottle Mosaic Virus |
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| Abstract: | Abstract: The RT-PCR and sequencing methods were used to identify whether cucumber green mottle mosaic viruses (CGMMV) exist in Myzus persicae (sulzer). M. persicae field population of Ningxiang, Chenzhou and Liuyang tobacco-growing area in Hunan province were collected in this study. After their RNAs were extracted, they were all reversely transcribed into cDNA. They were PCR amplified by taking cDNA as a template via designed primers and then the sequencing was carried out after shaking bacteria bottles. The sequencing results showed that the RNA virus was amplified, which was similar as to the cucumber green mottle mosaic virus up to 99%. The nucleotide sequences of 22 genes including this isolate were compared and the phylogenetic tree was constructed. CGMMV geographical source factors were combined to analyze the problem. The results showed that CGMMV existed in M. persicae, which came from the CGMMV carried on its own. M. persicae could spread the cucumber green mottle mosaic virus that was speculated through the analysis and the spread way of which was persistent. |
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| Keywords: | Myzus persicae Cucumber green mottle mosaic virus tTransmission wWay |
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