| 转iaaM基因陆地棉种质系的经济性状及其杂种F1表达特征 |
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| 引用本文： | 陈旭升,狄佳春,赵亮.转iaaM基因陆地棉种质系的经济性状及其杂种F1表达特征[J].中国农学通报,2018,34(25):21-25. |
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| 作者姓名： | 陈旭升 狄佳春 赵亮 |
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| 作者单位： | 江苏省农业科学院经济作物研究所,江苏省农业科学院经济作物研究所,江苏省农业科学院经济作物研究所 |
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| 基金项目： | 转基因生物新品种培育科技重大专项“长江下游棉区高产高效转基因棉花新品种培育”(2016ZX08005001-008)；转基因生物新品种培育科 技重大专项“特色专用转基因棉花新品种培育”(2016ZX08005-005)。 |
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| 摘 要： | 为阐明转iaaM 基因陆地棉种质系在Ⅰ型高品质杂交棉育种中的利用价值，本研究采用4 个转iaaM 基因的陆地棉新种质系、2 个高品质陆地棉种质系，及其4 个杂交组合做PCR分子检测，并对其经济性状进行区组比较试验。结果表明：4 个转iaaM 基因的父本及其杂交F1在500 bp 处均出现特征主带，而2 个高品质母本无特征主带，显示iaaM 基因在杂交F1代呈显性表达。转iaaM 种质系的衣分较高、棉铃较大、单株结铃较多、脱落率较低；但籽指偏小、种子空瘪率较高。具有iaaM 基因的杂交F1组合，其衣分、单铃重、结铃数接近转iaaM 亲本，表明转iaaM 基因的产量性状三要素在F1代呈显性表达；其中有2个杂交组合的纤维品质达优质Ⅰ型，且1 个组合的皮棉产量与Ⅲ型杂交棉对照‘苏杂201’相比，仍具有正向竞争优势。利用iaaM 种质系做为杂交亲本，只要杂交配组适当，培育具有产量优势的Ⅰ型高品质陆地杂交棉在实践上是完全可行的。
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| 关 键 词： | 组织染色法 组织染色法 西瓜 硼胁迫 死亡细胞 逆境生理 |
| 收稿时间： | 2017/6/19 0:00:00 |
| 修稿时间： | 2018/8/13 0:00:00 |
Economic Characters and Hybrid F1 Expressing Characteristics of iaaM-transgenic Upland Cotton Germplasms |
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| Abstract: | To illuminate the value of iaaM-transgenic upland cotton in cultivating Ⅰ type of high quality hybrid cotton, PCR was used for the molecular detection of four iaaM-transgenic upland cotton germplasms, two upland cotton germplasms with high fiber quality and four F1 hybrids. A block comparison test was conducted to analyze the economic characters. The results showed that four iaaM-transgenic male parents and their hybrid F1 appeared characteristic main band in 500 bp, but two female parents had no characteristic main band, which indicated that iaaM gene was dominant expression in hybrid F1 generation. The iaaM-transgenic upland cotton germplasms had higher lint percentage, bigger boll weight, more bolls per plant and lower abscission rate, but with smaller seed index and higher empty seed rate. The lint percentage, boll weight and
boll number of hybrid F1 with iaaM gene were close with the iaaM-transgenic parents, which indicated that the three factors with iaaM-transgenic yield characters were dominant expression in hybrid F1. The fiber quality of two hybrid combinations reached Ⅰ type standard, and one combination still had positive competitive advantage in lint yield, compared with Ⅲ type contrast‘Suza201’. When iaaM germplasm is used as cross parent, if we choose the suitable cross matching, it is feasible in practice for breeding Ⅰ type of high quality hybrid cotton with yield competitive advantage. |
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| Keywords: | Upland cotton iaaM gene Economic character Heterosis |
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