| 中国绥芬河三块鱼不同群体的种属划分及起源 |
| |
| 引用本文： | 常玉梅,程磊,孙博,苏宝锋,梁利群,王维坤.中国绥芬河三块鱼不同群体的种属划分及起源[J].中国水产科学,2018,25(4):811-818. |
| |
| 作者姓名： | 常玉梅 程磊 孙博 苏宝锋 梁利群 王维坤 |
| |
| 作者单位： | 淡水鱼类育种国家地方联合工程实验室农业农村部淡水水产生物技术与遗传育种重点实验室中国水产科学研究院特殊生境鱼类种质特性与抗逆育种重点实验室中国水产科学研究院黑龙江水产研究所;黑龙江省东宁市水产局 |
| |
| 基金项目： | 科技部科技基础性工作专项（2013FY110700）；中国水产科学研究院中央级公益性科研院所基本科研业务费专项（2014A11JC04）. |
| |
| 摘 要： | 为进一步明确中国绥芬河三块鱼属(Tribolodon)鱼类的分类地位及其起源,本研究依据表型和洄游产卵时间,将珠星三块鱼(Tribolodon hakonensis)、三块鱼(T.brandtii)及二者疑似杂交种与日本珠星三块鱼共53尾样本的COI基因序列进行了分析和比较。COI有效序列长度为637 bp,共检测到8种单倍型,其中三块鱼独享4种、珠星三块鱼独享3种、日本珠星三块鱼独享1种;疑似杂交种虽与三块鱼共享2种单倍型,却有80%的个体的单倍型与珠星三块鱼主效单倍型一致。系统进化分析显示,珠星三块鱼与珠星三块鱼南方型(sourthern forms of T.hakonensis)聚为一支,日本珠星三块鱼与珠星三块鱼北方型(northern forms of T.hakonensis)聚为一支,三块鱼(T.brandtii)单独聚为一支,由此可以确定日本海大彼得湾经俄罗斯滨海区溯河到中国河区产卵的三块鱼实为珠星三块鱼南方型和三块鱼。遗传距离结果显示,珠星三块鱼南方型和北方型的遗传距离为0.023,符合COI类群间的划分依据(0.02),支持将它们划分为两个有效种或亚种更妥当。依据COI分析结果,推测珠星三块鱼南方型和三块鱼存在杂交的可能性,提出了三块鱼野生种质资源保护和合理利用的相关建议。
|
| 关 键 词： | 绥芬河 三块鱼 杂交种 COI基因 系统进化 |
| 修稿时间： | 2018/8/20 0:00:00 |
Species classification and origin of Tribolodon in the Suifen River, China |
| |
| CHANG Yumei,CHENG Lei,SUN Bo,SU Baofeng,LIANG Liqun,WANG Weikun.Species classification and origin of Tribolodon in the Suifen River, China[J].Journal of Fishery Sciences of China,2018,25(4):811-818. |
| |
| Authors: | CHANG Yumei CHENG Lei SUN Bo SU Baofeng LIANG Liqun WANG Weikun |
| |
| Institution: | 1. National & Local United Engineering Laboratory of Freshwater Fish Breeding;Key Laboratory of Freshwater Aquatic Biotechnology and Genetic Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Fish Stress Resistance Breeding and Germplasm Characteristics on Special Habitats, Chinese Academy of Fishery Sciences;Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;2. Dongning Municipal Bureau of Fishery of Heilongjiang Province, Dongning 157200, China |
| |
| Abstract: | To further clarify the taxonomic status of Tribolodon species in the Suifen River in China, COI gene sequences of 53 samples including Tribolodon hakonensis, T. brandtii, their suspected hybrids in Suifen River, and T. hakonensis, which was collected from Japan, were compared and analyzed. Eight haplotypes were obtained with an effective sequence length of 637 bp. Haplotype analysis showed that T. brandtii had four haplotypes, T. hakonensis had three haplotypes, whereas T. hakonensis collected from Japan had an independent haplotype. Although the suspected hybrids shared two haplotypes with their parents, 80% individuals shared the same main haplotype with T. hakonensis. Phylogenetic analysis using the neighbor-joining method based on the K2P model showed that T. hakonensis clustered with the southern forms of T. hakonensis in the southern region of Russia (i.e., Primorsky krai), T. hakonensis from Japan clustered with northern forms of T. hakonensis in the northern region of Russia (i.e. Sakhalin Island and Khabarovski krai), and T. brandtii clustered singly. Therefore, anadromous spawning populations of Tribolodon species from the Japan Sea to the Suifen River actually are the southern forms of T. hakonensis and T. brandtii, respectively. The average genetic distance between the southern and northern forms of T. hakonensis was 0.023, conforming to the basis of segregation of COI between groups (>0.02). We support the classification of the southern and northern forms of T. hakonensis into two effective species or subspecies. Based on the results of the COI analysis, we speculate that hybrids of T. hakonensis and T. brandtii were probably produced in nature or by artificial propagation, and therefore, suggestions were proposed on how to protect and utilize the fish germplasm resources rationally. |
| |
| Keywords: | Suifen River Tribolodon species hybrid COI gene phylogeny |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国水产科学》浏览原始摘要信息 |
| 点击此处可从《中国水产科学》下载免费的PDF全文 |
|
