| 冬瓜EMS诱变条件优化及突变体筛选 |
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| 引用本文： | 弭宝彬,谢玲玲,王鑫,张竹青,马艳青,周火强.冬瓜EMS诱变条件优化及突变体筛选[J].中国农学通报,2018,34(21):35-41. |
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| 作者姓名： | 弭宝彬 谢玲玲 王鑫 张竹青 马艳青 周火强 |
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| 作者单位： | 湖南省农业科学院蔬菜研究所 |
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| 基金项目： | 农业部农业科研杰出人才培养计划项目“农业部瓜果类蔬菜创新团体”；国家大宗蔬菜产业体系“长沙综合试验站”(CARS-23-G29)；湖南 省重点研发计划“优质、耐储运专用型瓜类蔬菜新品种选育及产业化”(2017NK2061)。 |
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| 摘 要： | 为研究甲基磺酸乙酯(EMS)诱变技术在冬瓜种质资源创制中的效果和探索适宜的冬瓜种子诱变体系,采用EMS磷酸缓冲液对冬瓜种子进行诱变处理,研究了处理浓度和诱变处理时间对冬瓜种子萌发和生长的影响,并对冬瓜M1、M2代突变体的田间生物学性状进行调查分析。结果表明,最佳的诱变条件为1.2%EMS诱变12 h,在此条件下,冬瓜种子发芽率、成苗率分别为54.67%、51.83%,接近半数致死浓度;在该诱变条件下大规模处理冬瓜种子10000粒,催芽播种4752粒,成苗4500株,植株具有典型的EMS处理后的真叶畸形、黄化等特征,证明EMS诱导成功,自交授粉获得586个自交瓜。种植M2代株系后获得叶、花、果实和株型等相关的突变体,突变率分别为27.22%、26.80%、20.47%、16.06%,冬瓜总突变频率为11.53%。本研究建立了冬瓜种子EMS诱变体系,构建了冬瓜突变体库,为冬瓜功能基因研究奠定了材料基础。
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| 关 键 词： | 冬瓜 EMS 变异性状 诱变条件 突变体库 |
| 收稿时间： | 2018/1/25 0:00:00 |
| 修稿时间： | 2018/7/8 0:00:00 |
EMS Mutagenesis Optimization and Mutants Screening of Wax Gourd |
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| Abstract: | To study the effect of ethyl methane sulfonate (EMS) induced mutation technology in the germplasm resources creation of wax gourd and to explore a suitable wax gourd seed induced mutation system, wax gourd seeds were subjected to mutagenesis using EMS phosphate buffer solution, and the effects of the treatment concentration and treating time on the wax gourd seed germination and growth were investigated. Moreover, the biological characters of M1 and M2 mutants were studied and analyzed. The results showed that the optimized induction condition was 1.2% EMS for 12 h. Under this condition, the seed germination frequency and seedling frequency of wax gourd were 54.67% and 51.83%, respectively, close to the lethal dose of 50%. And then 10000 wax gourd seeds were treated with this condition, 4752 seeds were sowed and pregerminated and 4500 converted into plant. The plants had the typical characteristics such as true leaf malformation and yellowing after EMS treatment, it proved that EMS induction was successful and 586 self- crossed wax gourds were obtained by self-pollination. After planting M2 strains, related mutants such as leaves, flowers, fruits and plant types were obtained, and the mutation frequencies were 27.22%, 26.80%, 20.47% and 16.06%, respectively. The total mutation frequency of wax gourd was 11.53%. This study establishes the EMS mutagenesis system of wax gourd seed and constructs the mutant library of wax gourd seed, laying a material foundation for the functional gene research of wax gourd. |
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| Keywords: | Wax gourd EMS Variation character Mutagenesis condition Mutant library |
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