| 高效液相色谱法测定麻杏石甘颗粒中盐酸麻黄碱含量 |
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| 引用本文： | 米彦飞,侯丽丽,武晋孝,郭禹.高效液相色谱法测定麻杏石甘颗粒中盐酸麻黄碱含量[J].中国兽药杂志,2020,54(3):26-31. |
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| 作者姓名： | 米彦飞 侯丽丽 武晋孝 郭禹 |
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| 作者单位： | 山西省畜牧产品质量安全检验监测中心,,, |
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| 摘 要： | 为完善麻杏石甘颗粒质量标准,采用高液相色谱法对麻杏石甘颗粒中盐酸麻黄碱的含量测定进行研究。采用Thermo scientific BDS C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸溶液(含0.1%三乙胺)(3∶97),检测波长207 nm,流速1.0 mL/min,柱温30℃,进样量10μL。结果表明,盐酸麻黄碱进样浓度在5~60μg/mL范围内,峰面积与盐酸麻黄碱含量呈良好线性关系(R^2=0.9999)。样品平均回收率为96.62%(n=6),RSD为1.30%。本方法简便、准确度高、重复性好、耐用性强,为进一步提高该制剂的质量标准,保证临床效果,提供了检测依据。
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| 关 键 词： | 高效液相色谱法 麻杏石甘颗粒 盐酸麻黄碱 |
| 收稿时间： | 2019/11/18 0:00:00 |
| 修稿时间： | 2020/2/29 0:00:00 |
Determination of Ephedrine hydrochloride in Maxingshigan granules by HPLC |
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| MI Yan-fei,HOU Li-li,WU Jin-xiao,GUO Yu.Determination of Ephedrine hydrochloride in Maxingshigan granules by HPLC[J].Chinese Journal of Veterinary Drug,2020,54(3):26-31. |
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| Authors: | MI Yan-fei HOU Li-li WU Jin-xiao GUO Yu |
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| Institution: | Shanxi animal husbandry product quality safety inspection and monitoring center,,, |
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| Abstract: | In order to improve the quality standard of maxingshigan granules,the content of ephedrine hydrochloride in maxingshigan granules was determined by high liquid chromatography.Thermo scientific BDS C18 column(4.6 mm×250 mm,5μm)was used,the mobile phase was acetonitrile-0.1% phosphoric acid solution(containing 0.1% triethylamine)(3∶97),the detection wavelength was 207 nm,the flow rate was 1.0 mL/min,the column temperature was 30℃,and the sample volume was 10μL.The results showed that the injection concentration of ephedrine hydrochloride was within the range of 5~60μg/mL,the peak area had a good linear relationship with the content of ephedrine hydrochloride(R^2=0.9999).The average recovery was 96.62%(n=6),and the RSD was 1.30%.This method is simple,accurate repeatable,and strong durability,which provides a basis for further controlling the quality standard of maxingshigan granules. |
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| Keywords: | HPLC Maxingshigan granules ephedrine hydrochloride |
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