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| 商丘地区棉花黄萎菌的分离与鉴定 | |
| 引用本文： | 刘冬梅,丁锦平,陈静,李成伟,李付广.商丘地区棉花黄萎菌的分离与鉴定[J].江西农业学报,2009,21(8):116-120. |
| 作者姓名： | 刘冬梅 丁锦平 陈静 李成伟 李付广 |
| 作者单位： | 1. 商丘师范学院,生命科学系,植物与微生物互作重点实验室,河南,商丘,476000 2. 商丘师范学院,生命科学系,植物与微生物互作重点实验室,河南,商丘,476000;中国农业科学院,棉花研究所、农业部,棉花遗传改良重点开放实验室,河南,安阳,455004 3. 中国农业科学院,棉花研究所、农业部,棉花遗传改良重点开放实验室,河南,安阳,455004 |
| 基金项目： | 973重大基础研究项目，转基因生物新品种培育重大专项 |
| 摘 要： | 从河南商丘各县区采集棉花黄萎病病株,对其进行黄萎病菌的分离和鉴定。采用连续稀释法和单孢分离法分离得到16个单菌系,并以这些单菌系作为研究对象,利用真菌通用引物ITS1和ITS4对所分离的单菌系内转录间隔区(ITS)进行PCR扩增,得到543 bp大小的片段,经过克隆测序和在GenBank中进行BLASTn分析,与安阳棉花黄萎菌的ITS序列的同源性均达到98%以上,证明了这些片段来自大丽轮枝菌(Verticillium dahliae)的ITS区。同时将这些序列在NCB I的GenBank进行了登记,获得5个登记号:FJ715500、FJ715501、FJ715502、FJ715503、FJ715504。 |
| 关 键 词： | 棉花黄萎菌 分离 鉴定 ITS序列 |
Isolation and Identification of Cotton Verticillium Wilt Fungi in Shangqiu Area |
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| LIU Dong-mei,DING Jin-ping,CHEN Jing,LI Cheng-wei,LI Fu-guang.Isolation and Identification of Cotton Verticillium Wilt Fungi in Shangqiu Area[J].Acta Agriculturae Jiangxi,2009,21(8):116-120. | |
| Authors: | LIU Dong-mei DING Jin-ping CHEN Jing LI Cheng-wei LI Fu-guang |
| Institution: | 1.Key Laboratory of Plant-microbe Interactions;Department of Life Science;Shangqiu Normal University;Shangqiu 476000;China;2.Institute of Cotton Research;Chinese Academy of Agricultural Sciences;Key Laboratory of Cotton Genetic Improvement;Ministry of Agriculture;Anyang 455004;China |
| Abstract: | In this experiment,the pathogen of cotton Verticillium wilt was separated and identified from cotton diseased plants selected from Shangqiu area,and 16 single-strains were isolated by using dilution method and single-spore isolation method.Fungal universal primers ITS1 and ITS4 were used for single-strains' internal transcribed spacer(ITS) PCR amplification.The results showed the size of fragment was 543 bp.Analysis of BLASTn in GenBank showed that the similarity of the sequences was 98% to the pathogen of ... |
| Keywords: | Cotton Verticillium dahliae Isolation Identification ITS sequence |
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