| 微卫星DNA标记在蓝狐单亲亲权鉴定中的应用 |
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| 引用本文： | 白玉妍,刘思宇,唐澜,贾竞波,刘志平.微卫星DNA标记在蓝狐单亲亲权鉴定中的应用[J].经济动物学报,2010,14(3):133-138,153. |
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| 作者姓名： | 白玉妍 刘思宇 唐澜 贾竞波 刘志平 |
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| 作者单位： | 东北林业大学野生动物资源学院,哈尔滨,150040 |
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| 基金项目： | 东北林业大学本科生创新性实验项目 |
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| 摘 要： | 主要探讨微卫星DNA标记在蓝狐(Alopex lagopus)单亲亲权鉴定中的可行性,为蓝狐育种的系谱精确鉴定提供依据。选择15个多态信息含量较高的微卫星DNA位点,以6窝蓝狐(母本6只,子代61只)为试验材料,计算15个基因座位的等位基因频率、杂合度(H)、多态信息含量(PIC)、非父排除概率(EP)、累积非亲排除概率(CCE)、亲权指数(PI)和亲权相对机会(RCP)。结果表明:15个微卫星位点的累积非亲排除概率值(CCE)为0.999996。单亲鉴定的PI值为850.5213~966160,RCP为99.8826%~99.9999%。说明所选的15个微卫星位点可用于蓝狐单亲亲权鉴定,其判定成功率和准确率较高,结果可靠。
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| 关 键 词： | 微卫星 蓝狐 单亲亲权鉴定 |
Application of Microsatellite Makers for Motherless Parentage Testing in Alopex lagopus |
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| BAI Yu-yan,LIU Si-yu,TANG Lan,JIA Jing-bo,LIU Zhi-ping.Application of Microsatellite Makers for Motherless Parentage Testing in Alopex lagopus[J].Journal of Economic Animal,2010,14(3):133-138,153. |
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| Authors: | BAI Yu-yan LIU Si-yu TANG Lan JIA Jing-bo LIU Zhi-ping |
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| Institution: | BAI Yu-yan,LIU Si-yu,TANG Lan,JIA Jing-bo,LIU Zhi-ping(College of Wildlife Resource,Northeast Forestry University,Harbin 150040,China) |
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| Abstract: | This study was initiated to assess the feasibility of motherless parentage testing by microsatellites in order to give help for family accuracy determination in bluefox(Alopex lagopus).15 genotypes of the most polymorphic microsatellites loci were detected in 6 families(6 female parents,61 offsprings)of bluefox.Gene frequency,expected heterozygosity(H),polymotphism information content(PIC),Exclusion Probability(EP),Cumulative Chance of Exclusion(CCE),Paternity Index(PI),Relative Chance of Paternity(RCP)were... |
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| Keywords: | microsatellite Alopex lagopus motherless parentage testing |
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