Immunostimulatory effects of human recombinant interleukin-12 on peripheral blood mononuclear cells from normal dogs |
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| Authors: | Brenda S Phillips Marcia L Padilla Erin B Dickerson Mary J Lindstrom and Stuart C Helfand |
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| Institution: | Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 53706, USA. |
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| Abstract: | Interleukin-12 (IL-12) plays a pivotal role in regulating cellular immune responses involving autoimmunity, infectious disease, and cancer. Human recombinant (hr) IL-12 is being evaluated for therapy of human cancer. We investigated the potential of hrIL-12 to activate canine peripheral blood mononuclear cells (PBMC) using proliferation and cytotoxicity as readouts. Human rIL-12 caused increased proliferation of PBMC, and enhanced lysis of allogeneic canine tumor targets mediated by PBMC from normal dogs in vitro. In addition, antibody-dependent cellular cytotoxicity (ADCC) mediated by canine PBMC was enhanced by hrIL-12. These results indicate that hrIL-12 is recognized by canine immune cells, triggering a number of immune responses in canine PBMC, that may be important for immunotherapy of canine cancer. Information from this investigation provides impetus for evaluation of the effects of hrIL-12 on PBMC from tumor-bearing dogs and should be helpful in the development of hrIL-12 as an immune cell activator in vivo in the dog. |
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| Keywords: | Dog Interleukin-12 Peripheral blood mononuclear cells Cytotoxicity Antibody-dependent cellular cytotoxicity Proliferation |
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