| 草原红牛改良群体H FABP基因SNP及其与肉质性状的相关分析 |
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| 引用本文： | 文力正,赵玉民,姜昊,张国梁,戴立胜,赵志辉,陈承祯,张嘉保.草原红牛改良群体H FABP基因SNP及其与肉质性状的相关分析[J].中国畜牧兽医,2008,35(7):66-69. |
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| 作者姓名： | 文力正 赵玉民 姜昊 张国梁 戴立胜 赵志辉 陈承祯 张嘉保 |
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| 作者单位： | 1.吉林大学实验动物中心，长春 130062； 2.吉林省农业科学院，长春 130000；3.吉林大学畜牧兽医学院，长春 130062 |
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| 摘 要： | 试验采用PCR-SSCP的方法对含1/2利木赞血缘的改良草原红牛的H-FABP基因5’-调控区进行SNP分析，并分析其多态性与改良草原红牛肉质性状的相关性。结果表明：改良草原红牛H-FABP基因5’-调控区存在多态位点，在136位点发生1个T碱基的插入突变，在142位点，发生了G→A转换。相关分析结果显示剪切力在AA和AB、BB基因型间差异极显著（P<0.01），AB与BB基因型间差异不显著（P>0.05），A基因对剪切力有负效应，其余肉质性状在不同基因型间差异均不显著（P>0.05）。表明H FABP基因对改良草原红牛肉质嫩度有显著影响。
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| 关 键 词： | H FABP PCR SSCP SNP 肉质性状 草原红牛 |
Single Nucleltide Polymorphism of H FABP Gene and Correlation Analysis with Meat Quality Traits in Red Steppe Crossed Herds |
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| WEN Li-zheng,ZHAO Yu-min,JIANG Hao,ZHANG Guo-liang,DAI Li-sheng,ZHAO Zhi-hui,CHEN Cheng-zhen,ZHANG Jia-bao.Single Nucleltide Polymorphism of H FABP Gene and Correlation Analysis with Meat Quality Traits in Red Steppe Crossed Herds[J].China Animal Husbandry & Veterinary Medicine,2008,35(7):66-69. |
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| Authors: | WEN Li-zheng ZHAO Yu-min JIANG Hao ZHANG Guo-liang DAI Li-sheng ZHAO Zhi-hui CHEN Cheng-zhen ZHANG Jia-bao |
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| Institution: | 1. Laboratory Animal Center, Jilin University, Changchun 130062,China;2.Jilin Academy of Agriculture Sciences, Changchun 130000,China; 3. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062,China |
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| Abstract: | PCR-SSCP was used in this study to detect H-FABP 5’UTR polymorphisms in 16 Red Steppe hybrids crossed with Limousin. The correlationship was carried out between different genotype and meat quality.The results showed that 5’UTR of H-FABP gene exist polymorphisms in Red Steppe Crossed Herds, base T was inserted in 136 bp and the transformation of G→A in 142 bp. A genotype was negatively to the tenderness trait. The difference between AA with AB and BB was extremely significant (P<0.01) in tenderness, but AB and BB was no significant difference(P>0.05).The difference between genotype with the other meat quality was not significant (P>0.05). The effect was very significant of H-FABP gene to tenderness traits in Red Steppe Crossed Herds. |
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| Keywords: | H-FABP PCR SSCP SNP meat quality Red Steppe |
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