| 饲料碳水化合物水平及饥饿处理对斜带石斑鱼生长及葡萄糖耐受能力的影响 |
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| 引用本文： | 毛义波,刘泓宇,谭北平,董晓慧,杨奇慧,迟淑艳,章双.饲料碳水化合物水平及饥饿处理对斜带石斑鱼生长及葡萄糖耐受能力的影响[J].水产学报,2014,38(4):550-559. |
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| 作者姓名： | 毛义波 刘泓宇 谭北平 董晓慧 杨奇慧 迟淑艳 章双 |
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| 作者单位： | 广东海洋大学,广东海洋大学,广东海洋大学,广东海洋大学,广东海洋大学,广东海洋大学,广东海洋大学 |
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| 基金项目： | 国家自然科学基金;省自然科学基金;其它 |
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| 摘 要： | 为揭示斜带石斑鱼对碳水化合物利用的特点,研究了3个碳水化合物水平及饥饿处理对其生长、血浆生化指标及肝/肌糖原的影响。实验选取300尾初重为(35±0.28)g的幼鱼,设置持续投喂高(35%,CH)、中(21%,CM)、低(7%,CL)3个碳水化合物水平组,以及持续投喂组(CM)、饥饿再投喂组(R,饥饿4周+投喂4周,投喂CM组饲料)、饥饿组(S)3个投喂模式组。饲养8周后饥饿24 h,以30 mg/100 g体质量腹腔注射葡萄糖研究其代谢反应。结果显示,增重率和特定生长率随饲料碳水化合物水平的增加而升高,但无显著差异,饲料系数以CL组最高。不同碳水化合物水平下,各组注射葡萄糖后1~3 h血糖水平达峰值,但CM组在6 h内迅速回到注射前水平;各组血浆胰岛素水平均先降后升,但CL组在3 h后急剧下降;各组肝糖原、血浆甘油三酯含量在注射后1 h内均显著上升。饥饿处理下,持续投喂组(CM)血糖水平在6 h时迅速恢复至注射前水平;CM组和R组血浆胰岛素在1 h内显著下降,但S组持续上升;CM组血浆甘油三酯含量在0~6 h内显著高于其他两组。研究表明,饲喂中等水平碳水化合物(21%)比较符合斜带石斑鱼糖耐受能力,而饥饿处理下则以持续投喂组糖耐受能力最强。
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| 关 键 词： | 斜带石斑鱼 碳水化合物 营养状态 葡萄糖 糖耐量 |
| 收稿时间： | 2013/11/26 0:00:00 |
| 修稿时间： | 1/7/2014 12:00:00 AM |
Effects of different dietary carbohydrate levels and starvation on growth and glucose tolerance ability in grouper(Epinephelus coioides) |
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| MAO Yibo,LIU Hongyu,TAN Beiping,DONG Xiaohui,YANG Qihui,CHI Shuyan and ZHANG Shuang.Effects of different dietary carbohydrate levels and starvation on growth and glucose tolerance ability in grouper(Epinephelus coioides)[J].Journal of Fisheries of China,2014,38(4):550-559. |
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| Authors: | MAO Yibo LIU Hongyu TAN Beiping DONG Xiaohui YANG Qihui CHI Shuyan and ZHANG Shuang |
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| Institution: | Laboratory of Aquatic Animal Nutrition and Feed,College of Fisheries,Guangdong Ocean University,Laboratory of Aquatic Animal Nutrition and Feed,College of Fisheries,Guangdong Ocean University,Laboratory of Aquatic Animal Nutrition and Feed,College of Fisheries,Guangdong Ocean University,Laboratory of Aquatic Animal Nutrition and Feed,College of Fisheries,Guangdong Ocean University,Laboratory of Aquatic Animal Nutrition and Feed,College of Fisheries,Guangdong Ocean University,Laboratory of Aquatic Animal Nutrition and Feed,College of Fisheries,Guangdong Ocean University,Laboratory of Aquatic Animal Nutrition and Feed,College of Fisheries,Guangdong Ocean University |
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| Abstract: | The experiment was conducted to determine the effect of dietary carbohydrate levels(CBH) and starvation on the growth performance, biochemical indices in plasma and liver/muscle glycogen content of the grouper.Total 300 fish were randomly distributed into 5 treatments with triplicate groups of 20 fish(initial weight:35±0.28 g) in each treatment.Three isonitrogenous and isoenergetic diets were formulated containing three carbohydrate levels(35% CH, 21% CM, 7% CL), combined with starvation group(S) and refeeding group(R, starving 4W+refeeding 4W, 21% carbohydrate in the diet).Experiment lasted 8W.14 fish in each treatment were selected and injected with 300 mg glucose per 100 g body weight for glucose tolerance test(GTT) after the end of experiment and blood sampling at 0, 1, 3, 6, 12, 24, 48 h.The results showed that the weight gain and specific growth rate increased with dietary carbohydrate level increase but no significant difference(P>0.05), and the feed coefficient rate of CL group was the highest(P<0.05).Plasma glucose levels significantly increased in 1-3 h and then decreased in CH, CM and CL(P<0.05).Plasma glucose levels in CM returned to the initial values within 6 h after being injected and initial level of plasma glucose content in CH and CL were obtained within 12 h.Plasma insulin content in CH, CM and CL decreased at 1 h and then increased, but insulin level of CL group significantly decreased at 3 h(P<0.05).Glycogen content in liver and plasma triglyceride levels were significantly increased in CH, CM and CL within 1 h after injection(P<0.05).CM, S and R groups presented persistent hyperglycemia after being injected and CM restored initial level quickly within 6 h.Plasma insulin levels of CM and R groups significantly decreased within 1 h(P<0.05), but that of R group continued to rise within 12 h.Plasma triglyceride levels of CM were higher than those of S and R groups within 0 to 6 h(P<0.05).The results of GTT indicate that proper carbohydrate level in diet(21%) and continuous feeding can significantly improve glucose tolerance of the grouper. |
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| Keywords: | Epinphelus coioides Carbohydrate Nutrition status Glucose Glucose tolerance test |
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