| 杨梅超氧化物歧化酶SOD2同源基因片段的克隆及序列分析 |
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| 引用本文： | 董乐,王芳,戴聪杰,林娈,陈端玉,王毓媛,林静.杨梅超氧化物歧化酶SOD2同源基因片段的克隆及序列分析[J].广东农业科学,2011,38(19):124-127. |
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| 作者姓名： | 董乐 王芳 戴聪杰 林娈 陈端玉 王毓媛 林静 |
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| 作者单位： | 泉州师范学院化学与生命科学学院,福建泉州,362000 |
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| 基金项目： | 福建省教育厅科技计划项目(JA09212); 泉州市优秀人才培养专项(09A07); 福建省高校服务海西建设重点项目(A101); 福建省大学生创新性实验计划项目(2010CXSY16) |
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| 摘 要： | 根据植物超氧化物歧化酶(superoxide dismutase,SOD)的保守区设计特异性引物,通过RT-PCR技术,克隆杨梅叶超氧化物歧化酶保守区之间的cDNA序列.该序列长233 bp,编码一段由77个氨基酸残基组成的SOD保守区片段,该肽段具有Cu/Zn SOD的特征序列.序列同源性分析表明,该序列与Genba...
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| 关 键 词： | 杨梅 超氧化物歧化酶 基因克隆 序列分析 |
Cloning and sequence analysis of Cu/Zn superoxide dismutase gene 2(MrSOD2) cDNA from Myrica rubra |
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| DONG Le,WANG Fang,DAI Cong-jie,LIN Luan,CHEN Duan-yu,WANG Yu-yuan,LIN Jing.Cloning and sequence analysis of Cu/Zn superoxide dismutase gene 2(MrSOD2) cDNA from Myrica rubra[J].Guangdong Agricultural Sciences,2011,38(19):124-127. |
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| Authors: | DONG Le WANG Fang DAI Cong-jie LIN Luan CHEN Duan-yu WANG Yu-yuan LIN Jing |
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| Institution: | DONG Le,WANG Fang,DAI Cong-jie,LIN Luan,CHEN Duan-yu,WANG Yu-yuan,LIN Jing(School of Chemistry and Life Science,Quanzhou Normal College,Quanzhou 362000,China) |
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| Abstract: | A PCR-based homologous cloning strategy was used to identify superoxide dismutase(SOD) genes from the leaves of Myrica rubra.Results of sequence analysis indicated that a 233 bp cDNA with a conserved regions encoding a 77 amino acids actin ortholog was successfully cloned and characterized.The SOD contained the signature sequence of Cu/Zn-SOD gene family.Multiple alignment analysis of amino acid sequences of the putative SOD conserved regions in Myrica rubra was more than 86% homologous with the best scorin... |
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| Keywords: | Morella rubra superoxide dismutase(SOD) gene cloning sequence analysis |
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