| 稻田土壤DNA的提取及nifA基因的PCR扩增 |
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| 引用本文： | 魏志琴,宋培勇,杨秀荣,马莉莉.稻田土壤DNA的提取及nifA基因的PCR扩增[J].贵州农业科学,2011(11):126-128,132. |
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| 作者姓名： | 魏志琴 宋培勇 杨秀荣 马莉莉 |
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| 作者单位： | 遵义师范学院生物系;湄潭求是高级中学; |
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| 基金项目： | 贵州省科学技术基金项目“贵州石漠化环境中宏基因组文库的构建”(20082103) |
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| 摘 要： | 为弄清茅贡米土壤微生物群落结构和生物固氮等代谢活动机制,采用试剂盒、SDS高盐和土壤预处理+SDS高盐3种土壤DNA提取方法,以从贵州茅贡米基地采集的土样提取的宏基因组DNA为模板,进行nifA基因的PCR扩增,比较了3种土壤DNA提取方法的效果.结果表明,试剂盒提取的DNA扩增到约200 bp和900 bp的nifA...
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| 关 键 词： | 茅贡米 稻田土壤 DNA提取方法 宏基因组 nifA基因 聚合酶链式反应 |
Extraction of DNA from Soils of Paddy Fields and PCR Amplification of nifA Gene |
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| WEI Zhi-qin,SONG Pei-yong ,YANG Xiu-rong,MA Li-li.Extraction of DNA from Soils of Paddy Fields and PCR Amplification of nifA Gene[J].Guizhou Agricultural Sciences,2011(11):126-128,132. |
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| Authors: | WEI Zhi-qin SONG Pei-yong YANG Xiu-rong MA Li-li |
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| Institution: | WEI Zhi-qin1,SONG Pei-yong1 *,YANG Xiu-rong1,MA Li-li2(1.Department of Biology,Zunyi Normal College,Zunyi,Guizhou 563002,2.Qiushi Senior High School,Meitan,Guizhou 564100,China) |
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| Abstract: | Three methods of DNA extraction from soils of paddy fields for production of Maogong rice variety were compared by PCR amplification of nifA gene taking DNA of metagenome as the template to study the community structure of soil microorganism and metabolic mechanism of biological nitrogen fixation.The results showed that the nifA gene segments with 200 bp and 900 bp could be amplified from the extracted DNA by a kit,but the nifA gene segments with 200 bp were amplified from the extracted DNA by SDS and pre-t... |
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| Keywords: | Maogong rice paddy soil DNA extraction method metagenome nifA gene PCR |
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