| 溶藻细菌W-04的筛选及溶藻效果初探 |
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| 作者姓名： | 王海玉 赵 芳 彭 谦 孙珮石 |
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| 作者单位： | 王海玉,赵芳,孙佩石(云南大学工程技术研究院,昆明,650091);彭谦(云南大学微生物研究所,昆明,650091)? |
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| 基金项目： | 云南大学校级科研项目"微生物法清除滇池水厂藻渣的研究"? |
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| 摘 要： | 为有效治理滇池藻类大量繁殖对水体和环境的严重污染,利用筛选果胶分解菌的培养基从滇池水样中筛选到的1株红色细菌(编号为W-04),用W-04是以果胶作为唯一碳源的细菌.用W-04与铜绿微囊藻(M.aeruginosa Kütz)共培养,发现藻细胞数量与加入的菌液及无菌滤液的量呈负相关关系,菌藻液体(带菌或无菌滤液)体积比为10/100的试验组藻细胞第8天时残存数为0,连续培养30天叶绿素测定结果为0,经显微观察藻细胞数也为0,表明藻体包括叶绿素已完全降解,无再度繁殖能力.实验结果支持生物法治理滇池藻类过量繁殖的方案.
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| 关 键 词： | 筛选 细菌 铜绿微囊藻 溶藻效果 |
Isolation of an algae-constraining bacteria strain W-04 and its effects on the growth of M. aeruginosa Kütz |
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| Abstract: | A red bacterium named w-04 that could use pectin as the only carbon source was isolated from Dian-chi Lake. When cultivated with M. aeruginosa Kütz, the number of M. aeruginosa Kütz cells showed negative dependence on the amount of bacteria added. The number of M. aeruginosa Kütz cells changed periodically with the time of cultivation. The duration of the period was about 6 days. With the experimental group in which the ratio of liquid volume of bacteria to M. aeruginosa Kütz was 10 to 100, the viable cells of M. aeruginosa Kütz droped to 0 after 8-day cultivation. No chlorophyll was detected in the culture after 30-day continuous cultivation, indicating the M. aeruginosa Kütz cells, as well as chlorophyll, were broken down and could not propagate anymore. |
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