| Molecular Characterization of Atlas 66-Derived Wheat Near-lsogenic Lines Contrasting in Aluminum （Al） Tolerance |
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| 作者姓名： | GUO Pei-guo BAI Gui-hua LI Rong-hua Brett Carver Michael Baum |
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| 作者单位： | [1]College of Life Science, Guangzhou University, Guangzhou 510006, P.R.China [2]USDA-ARS-PIant Science and Entomology Research Unit, 4008 Throckmorton Hall, Kansas State University, Manhattan, KS 66506, USA [3]Department of Plant and Soil Sciences, Oklahoma State University, 0K74078, USA [4]International Center for Agricultural Research in the Dry Areas （ICARDA）, P.O.Box 5466, Aleppo, Syria |
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| 摘 要： | Aluminum （Al） toxicity is the major limiting factor for wheat growth in acidic soils. Genetic improvement of Al tolerance is one of the most cost-effective solutions to improve wheat productivity. The objective of this study was to characterize near isogenic lines （NILs） contrasting in Al tolerance derived from Atlas 66 in the backgrounds of Al-sensitive cultivars Chisholm and Century using amplified fragment length polymorphism （AFLP） and simple sequence repeat （SSR）. A total of 200 AFLP and 88 SSR primer pairs were screened and 12 markers （11 AFLPs and one SSR） were associated with Al-tolerance in NILs of at least one recurrent parental background. Among them, nine were linked to A1 tolerance in the Chisholm-derived NILs, seven were associated with Al-tolerance in the Century-derived NILs, and three AFLPs derived from the primer combinations of pAG/mGCAG, pCAG/mAGC and pGTG/mGCG, and one SSR, Xwmc331 on chromosome 4D, associated with A1 tolerance in NILs of both recurrent parental backgrounds. Those common markers across two backgrounds may be the major marker loci associated with Al-tolerance in Atlas 66 and could be useful for marker-assisted breeding to improve Al tolerance in wheat. In addition, evaluation of Al tolerance among different genotypes using hematoxylin stain and relative root growth revealed that Atlas 66 was more tolerant to Al stress than the NILs, therefore suggested that the Al-tolerant NILs might not carry all Al-tolerance loci from Arias 66 and inheritance of Al tolerance in Arias 66 is more likely multigenic.
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| 关 键 词： | 小麦 近等基因系 Atlas66 铝耐受性 分子特征 分子标记 |
| 收稿时间： | 2006-10-31 |
| 修稿时间： | 2007-03-26 |
Molecular Characterization of Atlas 66-Derived Wheat Near-Isogenic Lines Contrasting in Aluminum (Al) Tolerance |
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| GUO Pei-guo BAI Gui-hua LI Rong-hua Brett Carver Michael Baum.Molecular Characterization of Atlas 66-Derived Wheat Near-Isogenic Lines Contrasting in Aluminum (Al) Tolerance[J].Agricultural Sciences in China,2007,6(5):522-528. |
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| Authors: | GUO Pei-guo BAI Gui-hua LI Rong-hua Brett Carver Michael Baum |
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| Institution: | 1. Biotechnology Research Institute/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, China;2. Shenzhen Nongke Group Co., Ltd., Shenzhen, China;1. The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;2. College of Agronomy, State Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, Henan, China;3. Department of Biosciences, COMSATS Institute of Information Technology, Sahiwal Campus, Pakistan;1. Department of Biotechnology, Lovely Professional University, Phagwara, India;2. Punjab Biotechnology Incubators, Mohali, India;3. Regional Advanced Water Testing Laboratory, Mohali, India;4. Regional Ayurveda Research Institute for Drug Development, Gwalior, India;5. Department of Zoology, Lovely Professional University, Phagwara, India |
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| Abstract: | Aluminum (Al) toxicity is the major limiting factor for wheat growth in acidic soils. Genetic improvement of Al tolerance is one of the most cost-effective solutions to improve wheat productivity. The objective of this study was to characterize near isogenic lines (NILs) contrasting in Al tolerance derived from Atlas 66 in the backgrounds of Al-sensitive cultivars Chisholm and Century using amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR). A total of 200 AFLP and 88 SSR primer pairs were screened and 12 markers (11 AFLPs and one SSR) were associated with Altolerance in NILs of at least one recurrent parental background. Among them, nine were linked to Al tolerance in the Chisholm-derived NILs, seven were associated with Al-tolerance in the Century-derived NILs, and three AFLPs derived from the primer combinations of pAG/mGCAG, pCAG/mAGC and pGTG/mGCG, and one SSR, Xwmc331 on chromosome4D, associated with Al tolerance in NILs of both recurrent parental backgrounds. Those common markers across two backgrounds may be the major marker loci associated with Al-tolerance in Atlas 66 and could be useful for marker-assisted breeding to improve Al tolerance in wheat. In addition, evaluation of Al tolerance among different genotypes using hematoxylin stain and relative root growth revealed that Atlas 66 was more tolerant to Al stress than the NILs, therefore suggested that the Al-tolerant NILs might not carry all Al-tolerance loci from Atlas 66 and inheritance of Al tolerance in Atlas 66 is more likely multigenic. |
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| Keywords: | aluminum (Al) tolerance Triticum aestivum L molecular marker |
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