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NaCl预处理对盐胁迫下水稻H2O2含量和过氧化氢酶活力的影响
引用本文:高宇,徐春莹,王丹,周燕,张亚玲,林志伟,张兴,靳学慧.NaCl预处理对盐胁迫下水稻H2O2含量和过氧化氢酶活力的影响[J].安徽农业科学,2014,42(36):12814-12815,12828.
作者姓名:高宇  徐春莹  王丹  周燕  张亚玲  林志伟  张兴  靳学慧
作者单位:黑龙江八一农垦大学农学院,黑龙江大庆163319;黑龙江省科学院大庆分院,黑龙江大庆163712;黑龙江八一农垦大学农学院,黑龙江大庆,163319;黑龙江省科学院大庆分院,黑龙江大庆,163712
摘    要:目的]通过对水稻苗前期盐的处理后,测定盐胁迫条件下生理指标的变化,确定盐害对水稻的影响.方法]测定了一种盐敏感水稻品种(丽江新团黑谷)经过低浓度盐胁迫预处理后在盐害条件下叶片和根中活性氧H2O2的产生和CAT活力.结果]50 mmol/L盐胁迫能导致其叶片和根中H2O2含量显著上升,而CAT活力则显著下降.经过低浓度盐(20 mmol/L)预先处理的水稻在盐胁迫下H2O2含量上升幅度则较小,而CAT活力较高.此外,盐胁迫预处理的水稻在盐害条件下生长情况更好,其叶片中叶绿素含量更高.结论]活性氧H2O2及其清除酶CAT参与水稻对盐害的应答反应,并且这种预处理能增强水稻抗盐害胁迫能力.

关 键 词:水稻  盐害  过氧化氢  过氧化氢酶  预处理

Effects of NaCl Pretreatment on H2O2 Content and Catalase Activity of Rice under Salt Stress
Institution:GAO Yu, XU Chun-ying, WANG Dan, JIN Xue-hui ( 1. Agricuhural College, Heilongjiang Bayi Agricultural University, Daqing ,Heilongjiang 163319; 2. Heilongjiang Daqing Academy of Science, Daqing, Heilongjiang 163712)
Abstract: Objective] In order to study the effects of salt injury to rice, based on early rice seedhngs after pretreatment of salt, the changesof physiological indexes under salt stress were determine. Method] The salt sensitive rice variety of LTH was pretreated by low concentrationof salt stress. And H202 content and catalase activity in the leaves and the roots were studied. Result] 50 mmol/L salt stress could signifi-cantly increase H202 content in the leaves and the roots, but significantly decrease CAT activity. The rise of H202 content of rice pretreated bylow concentration of salt stress (20 mmol/L) was low, hut that of CAT activity was high. Otherwise, the growth of the rice pretreated by saltstress under the condition of salt stress was good, and chlorophyll content in the leaves was higher. Conclusion] H202 and CAT were engagedin the response reaction of rice to salt stress. And the pretreatment could improve the salt stress resistance of rice.
Keywords:Rice  Salt stress  Hydrogen peroxide  Catalase  Pretreatment
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