| 高等院校农林专业基因工程实验教学改革探索与实践 |
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| 引用本文： | 段永波,赵丰兰,宋运贤,盛玮,薛建平.高等院校农林专业基因工程实验教学改革探索与实践[J].安徽农业科学,2014(35):12780-12781. |
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| 作者姓名： | 段永波 赵丰兰 宋运贤 盛玮 薛建平 |
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| 作者单位： | 淮北师范大学生命科学学院,资源植物生物学安徽省重点实验室,安徽淮北235000 |
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| 基金项目： | 安徽省教育厅高校自然科学基金重点项目，资源植物生物学安徽省重点实验室开放项目，淮北师范大学细胞生物学精品课程建设项目 |
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| 摘 要： | 针对基因工程实验课程基于分子水平操作难以获得较好教学效果的问题,利用报告基因,以基因表达框的启动子和目的基因2个重要元件为切入点,在有限的教学课时内完成克隆真核生物基因启动子和报告基因并成功实现了报告基因在真核细胞中的表达.以此改变该课程主要以验证为主,且实验结果主要以单一的酶切图谱形式展示而不够直观的问题,进而提高了教学效果.
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| 关 键 词： | 基因工程实验 启动子 报告基因 生菜瞬时表达 |
Exploration and Practice of Genetic Engineering Experiments for Students in Agriculture and Forestry Colleges |
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| Institution: | DUAN Yong-bo, ZHAO Feng-lan, SONG Yun-xian et al ( Key Laboratory of Resource Plant Biology of Anhui Province, College of Life Sciences, Huaibei Normal University, Huaibei, Anhui 235000) |
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| Abstract: | Focusing on the trouble in getting satisfied teaching effectiveness in the course Genetic Engineering Experiments,we employed reporter gene in our teaching design.With two elements promoter and target gene required in any cassette as beginning point,we make the cloning of promoter and reporter gene and its expression in eukaryotic cells in limited class hours.It changed the previous teaching style of mainly for confirmation,and successfully overcame the shortages of nonintuitive presentation of experimental results singly by enzymatic pattern,improving the teaching effectiveness. |
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| Keywords: | Genetic Engineering Experiments Promoter Reporter gene Transient expression |
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