| 响应面法优化脚板薯皂苷提取工艺 |
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| 引用本文： | 刘媛洁,张良,刘芳兰,肖文烨.响应面法优化脚板薯皂苷提取工艺[J].安徽农业科学,2014,42(36):12885-12887. |
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| 作者姓名： | 刘媛洁 张良 刘芳兰 肖文烨 |
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| 作者单位： | 江西医学高等专科学校,江西上饶,334000;江西省食品发酵研究所,江西宜春,336000 |
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| 摘 要： | 目的]优化脚板薯皂苷的最佳提取工艺.方法]探讨提取温度、提取时间、料液比和乙醇浓度对脚板薯皂苷提取率的单因素影响.在此基础上,采用响应面分析方法,进一步研究各自变量及其交互作用对脚板薯皂苷提取率的影响,并对其工艺参数进行优化.结果]脚板薯皂苷提取的最佳工艺条件为提取温度76℃、提取时间1.5h、乙醇浓度90％、料液比1：30（m/v）,在此最优工艺条件下,脚板薯皂苷提取率达0.291％.结论]该研究可为脚板薯皂苷的工业化生产提供理论指导.
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| 关 键 词： | 脚板薯 响应面分析 皂苷 提取 |
Optimization of Extraction of Total Saponins from Dioscorea alata Lirm.sp by Response Surface Analysis |
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| Institution: | LIU Yuan-jie, ZHANG Liang, LIE Fang-lan ( 1. Jiangxi Medical College, Shangrao, Jiangxi 334000 ; 2. Jiangxi Food Fermen- tation Institute, Yichun, Jiangxi 336000) |
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| Abstract: | Objective ] The Response Surface Methodology （RSM） was used to optimize the extraction process of total saponins from Di-oscorea alata Lirm. sp. Method ] The impact of extraction temperature, extraction time, ethanol concentration and solid-liquid ratio on theyield of diosgenin are investigated, based on which the response surface analysis is adopted to study the impact of each variable and their inter-action on yield of diosgenin and optimize the extraction parameter. Result ] It is found that optimal parameters for diosgenin extraction formDioscorea alata Lirm. sp were as follows extraction temperature 76℃, extraction time 1.5 h , ethanol concentration 90%, solid-liquid ratio1：30（m/v）. Conclusion] The research provides the theoretical guidance for the industrial production of diosgenin in Dioscorea alata Lirm.sp. |
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| Keywords: | Dioscorea alata Lirm sp Response Surface Analysis Saponins Extraction |
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