| 种子处理及GA3浓度对诱导三七实生苗影响研究 |
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| 引用本文： | 孙玉琴,杨莉,韦美丽,黄天卫,陈中坚,王朝梁.种子处理及GA3浓度对诱导三七实生苗影响研究[J].特产研究,2013(4):47-49. |
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| 作者姓名： | 孙玉琴 杨莉 韦美丽 黄天卫 陈中坚 王朝梁 |
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| 作者单位： | [1]文山学院文山三七研究院,云南文山663000 [2]文山华信三七股份有限公司,云南文山663000 |
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| 基金项目： | 社会发展计划项目（2009CDl44） |
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| 摘 要： | 以健康饱满成熟的三七种子为外植体，接种到不同处理的培养基上，诱导实生苗。结果表明，剥除三七种皮，用70％的乙醇浸泡1min，再用0．1％升汞消毒灭菌10min，无菌水冲洗4～5次，接种到MS＋2，4-D（1．0mg／L）＋KT（0．5mg／L）＋GA3（40mg／L）＋蔗糖（30g／L）＋琼脂粉（7g／L）的培养基（pH5．8）中，根、茎、叶生长表现良好，从而初步建立了三七实生苗组培体系。
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| 关 键 词： | 三七 种子 组织培养 |
Effects of Different Treatments and GA3 Concentration on Induction Seedling of Panax notoginseng |
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| Institution: | SUN Yu - qin1,2, YANG Li1, WEI Mei - li1, HUANG Tian - wei1, CHEN Zhong - jian1, WANG Chao - liang1,2 ( 1. Sanqi Research Institute, Wenshan University, Wenshan 663000, China; 2. Wenshan Huaxin Notoginseng Co. , Ltd, Wenshan 663000, China) |
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| Abstract: | Plantlet of Panax notog/nseng induced from health full mature seeds which were inoculated on the different medium for establishment of tissue culture system and induction of seedling. The results showed that peeling seeds which were quick dip in 0.1% mercuric chloride for 10 minutes after sterilization in 70% alcohol for 1 minute were incubated in Petri dishes containing MS medium with 1.0mg/L 2,4 - D,0.5mg/L KT,40mg/L GA3,30g/L sucrose and 7.0g/L gelrite under pH 5.8.And the seedling of notoginseng were growing well.A preliminary efficient system for inducing seedling in Panax notoginseng was established. |
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| Keywords: | Panax notoginseng seedling tissue culture |
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